PMID- 9733905
OWN - NLM
STAT- MEDLINE
DCOM- 19981116
LR  - 20190831
IS  - 0165-3806 (Print)
IS  - 0165-3806 (Linking)
VI  - 110
IP  - 1
DP  - 1998 Sep 10
TI  - Appearance and distribution of two Ca2+-binding proteins during development of 
      the cochlea in the musk shrew.
PG  - 7-19
AB  - In the developing cochlea of the musk shrew, Suncus murinus, the localization of 
      two Ca2+-binding protein, calbindin and calmodulin, which are thought to play 
      different roles in the nervous system, was examined during gestational and 
      postpartum periods. Calbindin is thought to play a Ca2+ buffering role, while 
      calmodulin activates other proteins. Cochleae from the musk shrews sacrificed 
      from gestational day (GD) 15 to postnatal day (PP) 9 and as adults, were 
      immunohistochemically analyzed. The localization and order of appearance of 
      calmodulin in sensorineural elements were similar to those of calbindin, except 
      for timing of appearance. Calmodulin-staining was recognized first in the spiral 
      ganglion neurons on GD21, followed by the inner hair cells (IHCs) on GD23 and 
      outer hair cells (OHCs) on GD26, while calbindin immunoreactivity in the spiral 
      ganglion neurons on GD19, the IHCs on GD21 and the OHCs on GD23. In hair cells, 
      during development, immunostaining of calbindin and calmodulin was initially seen 
      in the cytoplasm, followed by the cuticular plate. Cytoplasmic staining then 
      decreased in mature hair cells. Non-sensorineural components also showed 
      positivity for both calbindin and calmodulin. The lateral wall of the cochlear 
      duct was positive for calbindin, while the stria vascularis was positive for 
      calmodulin. Immunoreactivity for calbindin was present earlier than that of 
      calmodulin in sensorineural elements, suggesting that in the developing cochlea, 
      calbindin and calmodulin have different functions and that Ca2+ buffering 
      capacity, which is regulated by Ca2+ buffer proteins, such as calbindin, may be 
      required before trigger proteins, such as calmodulin, function.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Nomiya, S
AU  - Nomiya S
AD  - Department of Otorhinolaryngology, Okayama University Medical School, Shikata-cho 
      2-5-1, Okayama, 700-8558, Japan.
FAU - Nishizaki, K
AU  - Nishizaki K
FAU - Anniko, M
AU  - Anniko M
FAU - Karita, K
AU  - Karita K
FAU - Ogawa, T
AU  - Ogawa T
FAU - Masuda, Y
AU  - Masuda Y
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res Dev Brain Res
JT  - Brain research. Developmental brain research
JID - 8908639
RN  - 0 (Calbindins)
RN  - 0 (Calmodulin)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (S100 Calcium Binding Protein G)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Calbindins
MH  - Calmodulin/biosynthesis/*genetics
MH  - Cochlea/embryology/growth & development/*metabolism
MH  - *Embryonic and Fetal Development
MH  - *Gene Expression Regulation, Developmental
MH  - Nerve Tissue Proteins/genetics
MH  - Neurons/*metabolism
MH  - S100 Calcium Binding Protein G/biosynthesis/*genetics
MH  - Shrews
EDAT- 1998/09/12 00:00
MHDA- 1998/09/12 00:01
CRDT- 1998/09/12 00:00
PHST- 1998/09/12 00:00 [pubmed]
PHST- 1998/09/12 00:01 [medline]
PHST- 1998/09/12 00:00 [entrez]
AID - S016538069800087X [pii]
AID - 10.1016/s0165-3806(98)00087-x [doi]
PST - ppublish
SO  - Brain Res Dev Brain Res. 1998 Sep 10;110(1):7-19. doi: 
      10.1016/s0165-3806(98)00087-x.