PMID- 9734607
OWN - NLM
STAT- MEDLINE
DCOM- 19981117
LR  - 20171116
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 54
IP  - 3
DP  - 1998 Sep
TI  - In situ analysis of C-C chemokine mRNA in human glomerulonephritis.
PG  - 827-36
AB  - BACKGROUND: Glomerular and tubulointerstitial accumulations of macrophages and T 
      cells are a prominent feature of immune inflammatory glomerulonephritis. The C-C 
      family of chemokines are major mononuclear-cell chemoattractants and may be 
      central to the recruitment of these cells. METHODS: Using in situ hybridization 
      (ISH) we analyzed the expression of mRNA for the C-C chemokines monocyte 
      chemoattractant protein-1 (MCP-1), macrophage inflammatory protein-1alpha and 
      beta (MIP-1alpha, MIP-1beta) and RANTES in renal biopsy material from twenty 
      patients with glomerulonephritis. RESULTS: In overt inflammatory 
      glomerulonephritides, chemokine transcripts were differentially expressed by 
      glomerular and tubulointerstitial leukocyte infiltrates, glomerular parietal and 
      proximal tubular epithelial cells and endothelial cells. There was little 
      expression in minimal change nephropathy and normal tissue. Expression of 
      individual chemokines correlated with intrarenal T cell and macrophage 
      infiltrates. Combined immunohistochemistry and ISH demonstrated that 56.9% of 
      cells expressing MCP-1 mRNA were CD68+ve (monocytes/macrophages) and 53% of 
      infiltrating CD68 +ve cells were MCP-1 mRNA positive. CONCLUSIONS: These studies 
      indicate that the in situ production of C-C chemokines by resident and 
      infiltrating cells may play a crucial role in regulating macrophage and T-cell 
      recruitment in glomerulonephritis.
FAU - Cockwell, P
AU  - Cockwell P
AD  - CCRIS and Department of Pathology, Medical School, University of Birmingham, 
      Edgbaston, England, United Kingdom.
FAU - Howie, A J
AU  - Howie AJ
FAU - Adu, D
AU  - Adu D
FAU - Savage, C O
AU  - Savage CO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD3 Complex)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Chemokines, CC)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, CD/analysis
MH  - Antigens, Differentiation, Myelomonocytic/analysis
MH  - CD3 Complex/analysis
MH  - Chemokine CCL2/genetics
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CCL5/genetics
MH  - Chemokines, CC/*genetics
MH  - Female
MH  - Glomerulonephritis/*metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Macrophage Inflammatory Proteins/genetics
MH  - Male
MH  - Middle Aged
MH  - RNA, Messenger/*analysis
EDAT- 1998/09/12 00:00
MHDA- 1998/09/12 00:01
CRDT- 1998/09/12 00:00
PHST- 1998/09/12 00:00 [pubmed]
PHST- 1998/09/12 00:01 [medline]
PHST- 1998/09/12 00:00 [entrez]
AID - S0085-2538(15)30699-2 [pii]
AID - 10.1046/j.1523-1755.1998.00053.x [doi]
PST - ppublish
SO  - Kidney Int. 1998 Sep;54(3):827-36. doi: 10.1046/j.1523-1755.1998.00053.x.