PMID- 9735418
OWN - NLM
STAT- MEDLINE
DCOM- 19981209
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 13
IP  - 4
DP  - 1998 Oct
TI  - Antiproliferative action of tumor necrosis factor-alpha on MCF-7 breastcancer 
      cells is associated with increased insulin-like growth factor binding protein-3 
      accumulation.
PG  - 865-9
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a multifunctional cytokine involved in 
      host response to neoplasia. TNF-alpha has been shown to inhibit proliferation and 
      induce apoptosis of MCF-7 breast carcinoma cells. Insulin-like growth factors I 
      and II (IGF-I and IGF-II) are potent mitogens involved in growth regulation of 
      breast epithelial cells and are implicated in the pathophysiology of breast 
      cancer. Their bioactivity is strongly influenced by specific IGF-binding proteins 
      (IGFBPs). We report that accumulation of IGFBP-3 in the conditioned media of 
      MCF-7 cells is increased over control values in the presence of TNF-alpha. The 
      increased IGFBP-3 accumulation induced by TNF-alpha is correlated with increased 
      IGFBP-3 mRNA abundance. TNF-alpha also decreases IGF-I receptor levels in MCF-7 
      cells. Estradiol-stimulated MCF-7 cell proliferation is associated with reduced 
      IGFBP-3 accumulation, and we show that TNF-alpha attenuation of 
      estradiol-stimulated proliferation is associated with increased IGFBP-3 
      accumulation. Finally, we demonstrate that an IGFBP-3 antisense 
      oligodeoxynucleotide antagonizes TNF-alpha-induced inhibition of cell 
      proliferation and TNF-alpha-induced IGFBP-3 accumulation. These data strongly 
      suggest that IGFBP-3 plays a role in modulation of breast cancer cell 
      proliferation by TNF-alpha.
FAU - Rozen, F
AU  - Rozen F
AD  - Lady Davis Research Institute of the Jewish General Hospital and Departments of 
      Medicine and Oncology, McGill University, Montreal, Quebec, H3T 1E2, Canada.
FAU - Zhang, J
AU  - Zhang J
FAU - Pollak, M
AU  - Pollak M
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Insulin-Like Growth Factor Binding Protein 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 10028-17-8 (Tritium)
RN  - 4TI98Z838E (Estradiol)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Blotting, Northern
MH  - Breast Neoplasms/*metabolism/pathology
MH  - Cell Division/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/pharmacology
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Insulin-Like Growth Factor Binding Protein 3/*drug effects/genetics/metabolism
MH  - RNA, Messenger/drug effects/metabolism
MH  - Thymidine/metabolism
MH  - Tritium
MH  - Tumor Cells, Cultured/cytology/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1998/09/15 00:00
MHDA- 1998/09/15 00:01
CRDT- 1998/09/15 00:00
PHST- 1998/09/15 00:00 [pubmed]
PHST- 1998/09/15 00:01 [medline]
PHST- 1998/09/15 00:00 [entrez]
AID - 10.3892/ijo.13.4.865 [doi]
PST - ppublish
SO  - Int J Oncol. 1998 Oct;13(4):865-9. doi: 10.3892/ijo.13.4.865.