PMID- 9735881
OWN - NLM
STAT- MEDLINE
DCOM- 19990115
LR  - 20191102
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 73
IP  - 4
DP  - 1998 Jul
TI  - Ruthenium red preserves glycoprotein peplomers of C-type retroviruses for 
      transmission electron microscopy.
PG  - 222-7
AB  - Peplomers, the glycoprotein projections of the outer viral envelope, are 
      distinctive for many viruses. Peplomers of retroviral C-type particles are 
      fragile and are not preserved in standard preparations for transmission electron 
      microscopy of thin sections, whereas the peplomers of B- and D- type retroviruses 
      are usually preserved. Ruthenium red, extensively used in transmission electron 
      microscopy to enhance the preservation of glycosylated proteins, was used in the 
      preparation of three retrovirus-producing lymphoblastoid cell lines: murine SC-1 
      cells producing the C-type murine leukemia retrovirus LP-BM5 that causes 
      immunodeficiency, human DG-75 cells producing a murine leukemia retrovirus, and 
      human C5/MJ cells producing human T-cell lymphotropic virus type I (HTLV-I). 
      Fixation of cells was carried out with ruthenium red present in the 
      glutaraldehyde, osmium tetroxide, and the ethanol dehydration through the 70% 
      ethanol step. The detailed structure of peplomers of these three different 
      viruses was well preserved.
FAU - Fassel, T A
AU  - Fassel TA
AD  - Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226, USA.
FAU - Raisch, K P
AU  - Raisch KP
FAU - Chetty, N
AU  - Chetty N
FAU - Grossberg, S E
AU  - Grossberg SE
FAU - Kushnaryov, V M
AU  - Kushnaryov VM
LA  - eng
GR  - AI-32710/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (Coloring Agents)
RN  - 0 (Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 11103-72-3 (Ruthenium Red)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Coloring Agents
MH  - Glycoproteins/*analysis
MH  - Humans
MH  - Leukemia Virus, Murine/*chemistry/ultrastructure
MH  - Mice
MH  - Microscopy, Electron
MH  - *Ruthenium Red
MH  - Viral Envelope Proteins/*analysis
EDAT- 1998/09/15 00:00
MHDA- 1998/09/15 00:01
CRDT- 1998/09/15 00:00
PHST- 1998/09/15 00:00 [pubmed]
PHST- 1998/09/15 00:01 [medline]
PHST- 1998/09/15 00:00 [entrez]
AID - 10.3109/10520299809141113 [doi]
PST - ppublish
SO  - Biotech Histochem. 1998 Jul;73(4):222-7. doi: 10.3109/10520299809141113.