PMID- 9737699
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20190915
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 12
IP  - 9
DP  - 1998 Sep
TI  - High rate of chromosome abnormalities detected by fluorescence in situ 
      hybridization using BCR and ABL probes in adult acute lymphoblastic leukemia.
PG  - 1473-81
AB  - The value of dual-color fluorescence in situ hybridization (FISH) with BCR and 
      ABL probes for the detection of the Philadelphia (Ph) translocation and of other 
      alterations involving ABL and/or BCR was evaluated in adult acute lymphoblastic 
      leukemia (ALL). One hundred and four patients were studied prospectively using 
      interphase nuclei FISH, chromosome analysis (CA), and PCR assays for the chimeric 
      BRC/ABL transcript. FISH detected a Ph translocation in 24 cases (23.1%), as was 
      confirmed by CA and/or PCR. FISH revealed a false positive diagnosis of a Ph 
      translocation in four cases (5% false positive rate). Among 54 cases with 
      combined FISH, CA and PCR assays, FISH failed to establish a correct diagnosis in 
      3.7%, PCR in 5.6%, and CA in 7.4%. The combination of two screening methods led 
      to discrepant results in 9.3% (FISH + PCR), 11.1% (FISH + CA), or 13% (CA + PCR) 
      of the cases. In seven of 80 (8.8%) Ph-negative patients, gain of BCR and/or ABL 
      was identified. Overall, FISH detected alterations of the BCR and/or ABL genes 
      with an incidence of 29.8% of the current study. Due to the possibility of false 
      positive diagnosis of a Ph translocation using dual-color FISH the combination 
      with chromosome and/or RT-PCR analyses is recommended in adult ALL patients.
FAU - Rieder, H
AU  - Rieder H
AD  - Abteilung Klinische Genetik, Medizinisches Zentrum fur Humangenetik, 
      Philipps-Universitat, Marburg, Germany.
FAU - Bonwetsch, C
AU  - Bonwetsch C
FAU - Janssen, L A
AU  - Janssen LA
FAU - Maurer, J
AU  - Maurer J
FAU - Janssen, J W
AU  - Janssen JW
FAU - Schwartz, S
AU  - Schwartz S
FAU - Ludwig, W D
AU  - Ludwig WD
FAU - Gassmann, W
AU  - Gassmann W
FAU - Bartram, C R
AU  - Bartram CR
FAU - Thiel, E
AU  - Thiel E
FAU - Loffler, H
AU  - Loffler H
FAU - Gokbuget, N
AU  - Gokbuget N
FAU - Hoelzer, D
AU  - Hoelzer D
FAU - Fonatsch, C
AU  - Fonatsch C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*diagnosis
MH  - Chromosome Disorders
MH  - False Negative Reactions
MH  - Fusion Proteins, bcr-abl/analysis/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - *Philadelphia Chromosome
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prospective Studies
EDAT- 1998/09/16 00:00
MHDA- 1998/09/16 00:01
CRDT- 1998/09/16 00:00
PHST- 1998/09/16 00:00 [pubmed]
PHST- 1998/09/16 00:01 [medline]
PHST- 1998/09/16 00:00 [entrez]
AID - 10.1038/sj.leu.2401127 [doi]
PST - ppublish
SO  - Leukemia. 1998 Sep;12(9):1473-81. doi: 10.1038/sj.leu.2401127.