PMID- 9739013
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20131121
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 23
IP  - 2
DP  - 1998 Oct
TI  - A two-color BCR-ABL probe that greatly reduces the false positive and false 
      negative rates for fluorescence in situ hybridization in chronic myeloid 
      leukemia.
PG  - 109-15
AB  - The t(9;22) translocation resulting in the fusion of BCR and ABL genes is 
      pathognomonic in chronic myeloid leukemia (CML) and may be investigated at the 
      molecular level using fluorescence in situ hybridization (FISH). Two-color 
      BCR-ABL probes visualizing one fusion signal (1F FISH) have high false positive 
      rates (FPR) and false negative rates (FNR). The FPR is a result of the random 
      spatial association of probe signals within normal interphase cells so that some 
      cells appear to contain the BCR-ABL fusion gene. The FNR of 1F FISH probes 
      depends on the distance between the BCR and ABL probes hybridized to the BCR-ABL 
      fusion gene (< or =368 kb); the "gap" between the signals causing the cell to be 
      interpreted as normal. To overcome these difficulties, a two-color probe was 
      used, employing four yeast artificial chromosome (YAC) sequences that span the 
      breakpoint regions of the BCR and ABL genes and that visualize the two fusion 
      signals BCR-ABL and ABL-BCR in CML cells (2F FISH). The FNR for the 2F FISH 
      probes was assessed on clonal Ph+ granulocyte-macrophage-colony-forming cell 
      (CFU-GM) derived colonies and was reduced to 0.4% (2/450), compared with an FNR 
      of 13.5% (111/823) with 1F FISH. The FPR in normal mononuclear cells for the 2F 
      FISH was 0. 19 +/- 0.12% (3/1,700), whereas the FPR using 1F FISH was 4.5 +/- 
      2.3% (63/1,294). The 2F FISH can thus be used to evaluate very small frequencies 
      of BCR-ABL-positive and -negative interphase cells and may be of use in the 
      clinical monitoring of CML.
FAU - Grand, F H
AU  - Grand FH
AD  - Department of Haematology, Imperial College School of Medicine, Hammersmith 
      Hospital, London, United Kingdom.
FAU - Chase, A
AU  - Chase A
FAU - Iqbal, S
AU  - Iqbal S
FAU - Nguyen, D X
AU  - Nguyen DX
FAU - Lewis, J L
AU  - Lewis JL
FAU - Marley, S B
AU  - Marley SB
FAU - Davidson, R J
AU  - Davidson RJ
FAU - Goldman, J M
AU  - Goldman JM
FAU - Gordon, M Y
AU  - Gordon MY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - DNA Probes/*genetics
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Leukocyte Count
MH  - Leukocytes, Mononuclear/chemistry
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
EDAT- 1998/09/17 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/09/17 02:03
PHST- 1998/09/17 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/09/17 02:03 [entrez]
AID - 10.1002/(SICI)1098-2264(199810)23:2<109::AID-GCC3>3.0.CO;2-6 [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Oct;23(2):109-15.