PMID- 9739021
OWN - NLM
STAT- MEDLINE
DCOM- 19990413
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 23
IP  - 2
DP  - 1998 Oct
TI  - Detection of t(11;14) using interphase molecular cytogenetics in mantle cell 
      lymphoma and atypical chronic lymphocytic leukemia.
PG  - 175-82
AB  - The chromosomal translocation t(11;14)(q13;q32) fuses the IGH and CCND1 genes and 
      leads to cyclin D1 overexpression. This genetic abnormality is the hallmark of 
      mantle cell lymphoma (MCL), but is also found in some cases of atypical chronic 
      lymphocytic leukemia (CLL), characterized by a poor outcome. For an unequivocal 
      assessment of this specific chromosomal rearrangement on interphase cells, we 
      developed a set of probes for fluorescence in situ hybridization (FISH). Northern 
      blotting was performed for analysis of the cyclin D1 expression in 18 patients. 
      Thirty-eight patients, with either a typical MCL leukemic phase (17 patients) or 
      atypical CLL with an MCL-type immunophenotype, i.e., CD19-, CD5+, CD23-/low, 
      CD79b/sIgM(D)++, and FMC7+ (21 patients), were analyzed by dual-color interphase 
      FISH. We selected an IGH-specific BAC probe (covering the JH and first constant 
      regions) and a commercially available CCND1 probe. An IGH-CCND1 fusion was 
      detected in 28 of the 38 patients (17 typical MCL and 11 cases with CLL). Cyclin 
      D1 was not overexpressed in two patients with typical MCL and an IGH-CCND1 
      fusion. In view of the poor prognosis associated with MCL and t(11;14)-positive 
      CLL, we conclude that this set of probes is a valuable and reliable tool for a 
      rapid diagnosis of these entities.
FAU - Avet-Loiseau, H
AU  - Avet-Loiseau H
AD  - Laboratory of Hematology, University Hospital, Nantes, France.
FAU - Garand, R
AU  - Garand R
FAU - Gaillard, F
AU  - Gaillard F
FAU - Daviet, A
AU  - Daviet A
FAU - Mellerin, M P
AU  - Mellerin MP
FAU - Robillard, N
AU  - Robillard N
FAU - Bouyge, I
AU  - Bouyge I
FAU - Arcot, S
AU  - Arcot S
FAU - Batzer, M
AU  - Batzer M
FAU - Talmant, P
AU  - Talmant P
FAU - Harousseau, J L
AU  - Harousseau JL
FAU - Milpied, N
AU  - Milpied N
FAU - Bataille, R
AU  - Bataille R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA Probes)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 14/*genetics
MH  - Cyclin D1/genetics
MH  - DNA Probes
MH  - Female
MH  - Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/pathology
MH  - Lymphoma, Non-Hodgkin/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Translocation, Genetic/*genetics
EDAT- 1998/09/17 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/09/17 02:03
PHST- 1998/09/17 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/09/17 02:03 [entrez]
AID - 10.1002/(SICI)1098-2264(199810)23:2<175::AID-GCC11>3.0.CO;2-N [pii]
AID - 10.1002/(sici)1098-2264(199810)23:2<175::aid-gcc11>3.0.co;2-n [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Oct;23(2):175-82. doi: 
      10.1002/(sici)1098-2264(199810)23:2<175::aid-gcc11>3.0.co;2-n.