PMID- 9739110
OWN - NLM
STAT- MEDLINE
DCOM- 19981120
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 806
IP  - 1
DP  - 1998 Sep 21
TI  - Reserpine attenuates D-amphetamine and MDMA-induced transmitter release in vivo: 
      a consideration of dose, core temperature and dopamine synthesis.
PG  - 69-78
AB  - Amphetamine releases dopamine through a transporter-mediated mechanism. The 
      purpose of this report was to further our understanding of the intracellular pool 
      from which amphetamine releases dopamine: the cytoplasmic pool, the vesicular 
      pool, or both. Rats were treated with D-amphetamine (AMPH) (1.0 or 10.0 mg/kg) or 
      an amphetamine analog, methylenedioxymethamphetamine (MDMA) (2.0, 5.0, or 10.0 
      mg/kg). Pre-treatment with 10.0 mg/kg reserpine (18 h prior to AMPH or MDMA) 
      attenuated dopamine release for high and low AMPH doses; however the low-dose 
      effect showed borderline significance. Pre-treatment with 10.0 mg/kg reserpine 
      attenuated dopamine and serotonin release induced by MDMA. The dopamine effect 
      was seen at all three MDMA doses; the effect on serotonin was only measured at 
      the 10.0 mg/kg dose. Reserpine pre-treatment caused reductions in core body 
      temperature; heating the rats to normal body temperature for 3 h prior to AMPH or 
      MDMA, and during the 4 h post-treatment period partially reversed the 
      reserpine-induced attenuation of dopamine release. However, the intermediate 
      level of dopamine release for the reserpinized-heated animals was not 
      significantly different from either the reserpine group (not heated) or the AMPH 
      or MDMA alone groups. In a separate group of rats, the effects of reserpine and 
      reserpine+heat on dopamine synthesis were measured. DOPA accumulation after 
      treatment with the aromatic acid decarboxylase inhibitor NSD-1015 (100 mg/kg, 30 
      min before sacrifice), was greater in rats treated with reserpine compared to 
      controls; heating the reserpinized rats did not significantly alter the amount of 
      DOPA accumulation; however there was a trend towards further increase. These 
      results suggest that D-amphetamine releases dopamine that is stored in both 
      vesicles and the cytoplasm. Cooling may contribute to the attenuation of AMPH or 
      MDMA-induced dopamine release observed after reserpine; however, AMPH or MDMA 
      dependence upon vesicular stores most likely explains the diminished release 
      after reserpine. The attenuation of AMPH or MDMA-induced transmitter release by 
      reserpine is thought to be counteracted by a reserpine-induced replenishment of 
      stores. Therefore, all doses of D-amphetamine may use vesicular stores; the 
      degree to which new synthesis counteracts the vesicular depletion may be the 
      variable which differentiates low from high doses of D-amphetamine.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Sabol, K E
AU  - Sabol KE
AD  - University of Mississippi, Department of Psychology, 301 Peabody Bldg., 
      University, MS 38677, USA.
FAU - Seiden, L S
AU  - Seiden LS
LA  - eng
GR  - DA00085/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Dopamine Agents)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Serotonin Agents)
RN  - 8B1QWR724A (Reserpine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - TZ47U051FI (Dextroamphetamine)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Body Temperature/drug effects/physiology
MH  - Dextroamphetamine/*pharmacology
MH  - Dopamine/biosynthesis
MH  - Dopamine Agents/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Neurotransmitter Agents/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reserpine/*pharmacology
MH  - Serotonin Agents/pharmacology
EDAT- 1998/09/18 00:00
MHDA- 1998/09/18 00:01
CRDT- 1998/09/18 00:00
PHST- 1998/09/18 00:00 [pubmed]
PHST- 1998/09/18 00:01 [medline]
PHST- 1998/09/18 00:00 [entrez]
AID - S0006-8993(98)00720-3 [pii]
AID - 10.1016/s0006-8993(98)00720-3 [doi]
PST - ppublish
SO  - Brain Res. 1998 Sep 21;806(1):69-78. doi: 10.1016/s0006-8993(98)00720-3.