PMID- 9741385
OWN - NLM
STAT- MEDLINE
DCOM- 19981120
LR  - 20191102
IS  - 1056-8719 (Print)
IS  - 1056-8719 (Linking)
VI  - 39
IP  - 3
DP  - 1998 Apr
TI  - Nitrate and nitrite anion concentration in the intact cerebral cortex of preterm 
      and nearterm fetal sheep: indirect index of in vivo nitric oxide formation.
PG  - 125-8
AB  - Pregnant sheep with a microdialysis probe implanted in the fetal cerebral cortex 
      were used to determine if nitrate and nitrite anions (nitrate/nitrite) could be 
      quantitated in the microdialysate as an indirect index of in vivo nitric oxide 
      formation. Pregnant ewes (term, about 147 days) were surgically instrumented at 
      gestational day (GD) 90 (n = 3; preterm) and GD 121 (n = 3; nearterm). Three days 
      later, following an overnight probe equilibration period, five dialysate samples 
      were collected continuously on ice at 1-h intervals (infusion rate of 1 
      (microl/min). The nitrate/nitrite concentration was determined by reducing a 
      10-microl aliquot of each dialysate fraction with hot acidic vanadium followed by 
      chemiluminescence quantitation of the nitric oxide product. The lower limit of 
      quantitative sensitivity of the method is 25 picomoles. Nitrate/nitrite 
      concentration was 16.6+/-7.3 microM for the preterm fetus and 19.7+/-1.9 microM 
      for the nearterm fetus. The data demonstrate that nitrate/nitrite, as an index of 
      in vivo nitric oxide formation, can be quantitated in microdialysate samples 
      collected from the intact fetal sheep cerebral cortex.
FAU - Reynolds, J D
AU  - Reynolds JD
AD  - Department of Anesthesiology, Duke University Medical Center, Durham, North 
      Carolina 27710, USA. reyno010@mc.duke.edu
FAU - Zeballos, G A
AU  - Zeballos GA
FAU - Penning, D H
AU  - Penning DH
FAU - Kimura, K A
AU  - Kimura KA
FAU - Atkins, B
AU  - Atkins B
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pharmacol Toxicol Methods
JT  - Journal of pharmacological and toxicological methods
JID - 9206091
RN  - 0 (Anions)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Anions
MH  - Cerebral Cortex/*embryology/*metabolism
MH  - Female
MH  - Microdialysis
MH  - Nitrates/*metabolism
MH  - Nitric Oxide/*biosynthesis
MH  - Nitrites/*metabolism
MH  - Pregnancy
MH  - Sheep
EDAT- 1998/09/19 00:00
MHDA- 1998/09/19 00:01
CRDT- 1998/09/19 00:00
PHST- 1998/09/19 00:00 [pubmed]
PHST- 1998/09/19 00:01 [medline]
PHST- 1998/09/19 00:00 [entrez]
AID - S1056-8719(98)00007-0 [pii]
AID - 10.1016/s1056-8719(98)00007-0 [doi]
PST - ppublish
SO  - J Pharmacol Toxicol Methods. 1998 Apr;39(3):125-8. doi: 
      10.1016/s1056-8719(98)00007-0.