PMID- 9741546
OWN - NLM
STAT- MEDLINE
DCOM- 19990114
LR  - 20150901
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 13
IP  - 4
DP  - 1998 Aug
TI  - Cytokinetic pattern in the thoracic spinal cord of chick embryos (incubation day 
      5-13) using PCNA staining and TUNEL method.
PG  - 405-13
AB  - For the cytokinetic studies using spinal cords of chick embryos, chronological 
      patterns of cell proliferation and programmed cell death (apoptosis) should be 
      known. Information in the early stages of chick embryos is available while data 
      on later stages are seldom available. To investigate the chronological patterns 
      of cell proliferation and apoptosis in the thoracic spinal cord of normal chick 
      embryos on incubation day 5, 6, 8, 10 and 13 (Hamburger and Hamilton stage 
      26-40), proliferating cell nuclear antigen (PCNA) staining and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method were 
      used. Cell proliferation was active at the germinal layer on days 5 and 6. It 
      markedly declined on day 8 and became negligible on day 13. TUNEL-positive cells 
      were mainly found in the germinal layer, the ventrolateral part of the mantle 
      layer and the dorsal root ganglion. Compared to PCNA-positive cells, 
      TUNEL-positive cells were sparse, especially after day 10, when only a few 
      positive cells were scattered. These results will be used as a control data for 
      the studies such as an experimental research for neural tube defects.
FAU - Wang, K C
AU  - Wang KC
AD  - Division of Pediatric Neurosurgery, Seoul National University Children's 
      Hospital, Korea.
FAU - Park, I S
AU  - Park IS
FAU - Chi, J G
AU  - Chi JG
FAU - Lee, M S
AU  - Lee MS
FAU - Lee, Y J
AU  - Lee YJ
FAU - Cho, B K
AU  - Cho BK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Division
MH  - Chick Embryo
MH  - Proliferating Cell Nuclear Antigen/*metabolism
MH  - Spinal Cord/*cytology/embryology
MH  - Staining and Labeling
MH  - Thoracic Vertebrae/*cytology/embryology
PMC - PMC3054426
EDAT- 1998/09/19 00:00
MHDA- 1998/09/19 00:01
PMCR- 1998/08/01
CRDT- 1998/09/19 00:00
PHST- 1998/09/19 00:00 [pubmed]
PHST- 1998/09/19 00:01 [medline]
PHST- 1998/09/19 00:00 [entrez]
PHST- 1998/08/01 00:00 [pmc-release]
AID - 10.3346/jkms.1998.13.4.405 [doi]
PST - ppublish
SO  - J Korean Med Sci. 1998 Aug;13(4):405-13. doi: 10.3346/jkms.1998.13.4.405.