PMID- 9742929
OWN - NLM
STAT- MEDLINE
DCOM- 19981008
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 434
IP  - 3
DP  - 1998 Sep 4
TI  - Biosynthesis of D-aspartate in mammalian cells.
PG  - 231-5
AB  - In this communication, we demonstrate that D-aspartate (D-Asp) is synthesized in 
      pheochromocytoma cells (PC12). To our knowledge this is the first report of 
      biosynthesis of D-Asp in mammalian cells. Synthesis of D-Asp was demonstrated by 
      its time-dependent accumulation in the cell culture, and by the fact that this 
      accumulation was proportional to the number of inoculated cells. D-Asp in PC12 
      cells was identified by (i) co-elution with authentic D-Asp on two different HPLC 
      columns, an octadesyl silica column and a Pirkle-type chiral column, (ii) 
      reversed elution order of D-Asp and L-Asp on another Pirkle-type chiral column 
      with an opposite configuration, and (iii) sensitivity to D-Asp oxidase. In the 
      cells the amount of D-Asp was approx. 12-14% of total Asp and no other 
      investigated D-amino acid was detected. The amount of D-Asp did not increase 
      during the culture of mouse 3T3 fibroblasts and human neuroblastoma NB-1 cells. 
      Immunocytochemical staining with anti-D-Asp antiserum demonstrated that D-Asp 
      synthesized is present in the cytoplasm of the cells.
FAU - Long, Z
AU  - Long Z
AD  - Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
FAU - Homma, H
AU  - Homma H
FAU - Lee, J A
AU  - Lee JA
FAU - Fukushima, T
AU  - Fukushima T
FAU - Santa, T
AU  - Santa T
FAU - Iwatsubo, T
AU  - Iwatsubo T
FAU - Yamada, R
AU  - Yamada R
FAU - Imai, K
AU  - Imai K
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 30KYC7MIAI (Aspartic Acid)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Aspartic Acid/*biosynthesis/chemistry/isolation & purification
MH  - Chromatography, High Pressure Liquid
MH  - Cytoplasm/chemistry
MH  - Humans
MH  - Mice
MH  - PC12 Cells
MH  - Rats
MH  - Stereoisomerism
MH  - Tumor Cells, Cultured
EDAT- 1998/09/22 00:00
MHDA- 1998/09/22 00:01
CRDT- 1998/09/22 00:00
PHST- 1998/09/22 00:00 [pubmed]
PHST- 1998/09/22 00:01 [medline]
PHST- 1998/09/22 00:00 [entrez]
AID - S0014579398009867 [pii]
AID - 10.1016/s0014-5793(98)00986-7 [doi]
PST - ppublish
SO  - FEBS Lett. 1998 Sep 4;434(3):231-5. doi: 10.1016/s0014-5793(98)00986-7.