PMID- 9743729
OWN - NLM
STAT- MEDLINE
DCOM- 19981005
LR  - 20031114
IS  - 0003-1488 (Print)
IS  - 0003-1488 (Linking)
VI  - 213
IP  - 6
DP  - 1998 Sep 15
TI  - Histologic features and results of virus isolation tests of tissues obtained from 
      teat lesions that developed in dairy cattle during winter.
PG  - 862-5
AB  - OBJECTIVE: To determine microscopic features and involvement of viruses in 
      teat-end lesions (TEL) of dairy cows during winter. SAMPLE POPULATION: Teats with 
      TEL on lactating Holstein cows and from udders of carcasses. PROCEDURE: Tissues 
      obtained from TEL of 10 teats from 7 cows on 2 research farms during the winter 
      of 1994 to 1995 and 13 teats with TEL excised from udders of carcasses at an 
      abattoir during February 1995 were submitted for virus isolation. During the 
      winter of 1995 to 1996, an increased prevalence of TEL was observed in a research 
      herd. After a decrease in ambient temperature, TEL were identified, and a 
      full-thickness section of epidermis was removed from skin surrounding teat 
      orifices. Tissues were examined by use of light and electron transmission 
      microscopy. RESULTS: Viruses were not isolated from TEL tissues. Lesions ranged 
      from mild elevations of the epidermis to thickened oval regions that encircled 
      the teat orifice. The most severe lesions were dark and had thick crusts. 
      Histologically, TEL were composed of thickened regions of epidermis most notably 
      caused by hyperplasia of cells within the stratum spinosum. Excess production of 
      keratinocytes was also evident, and the keratinocyte layer often contained 
      bacteria. Ultrastructurally, squamous cells contained large amounts of keratin, 
      but virions were not detected. Evidence of a viral etiologic agent for TEL was 
      not detected. CLINICAL IMPLICATIONS: Development of TEL may be associated with 
      decreases in ambient temperature. Numerous bacteria were evident in the keratin 
      of TEL. Lesions and associated bacteria may predispose cows to mastitis.
FAU - Timms, L L
AU  - Timms LL
AD  - Department of Animal Science, College of Veterinary Medicine, Iowa State 
      University, Ames 50011, USA.
FAU - Van der Maaten, M J
AU  - Van der Maaten MJ
FAU - Kehrli, M E Jr
AU  - Kehrli ME Jr
FAU - Ackermann, M R
AU  - Ackermann MR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Am Vet Med Assoc
JT  - Journal of the American Veterinary Medical Association
JID - 7503067
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cattle Diseases/pathology/*virology
MH  - Female
MH  - Mammary Glands, Animal/pathology/*virology
MH  - Mastitis, Bovine/etiology
MH  - Seasons
MH  - Virus Diseases/complications/pathology/*veterinary
EDAT- 1998/09/23 02:01
MHDA- 2001/03/28 10:01
CRDT- 1998/09/23 02:01
PHST- 1998/09/23 02:01 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/09/23 02:01 [entrez]
PST - ppublish
SO  - J Am Vet Med Assoc. 1998 Sep 15;213(6):862-5.