PMID- 9744559
OWN - NLM
STAT- MEDLINE
DCOM- 19981009
LR  - 20190826
IS  - 0009-2797 (Print)
IS  - 0009-2797 (Linking)
VI  - 114
IP  - 1-2
DP  - 1998 Jul 3
TI  - Inhibition of linoleic acid hydroperoxide-induced toxicity in cultured human 
      umbilical vein endothelial cells by catechins.
PG  - 109-19
AB  - The protective effect of catechins, major components of green tea, was studied in 
      cultured human umbilical vein endothelial cells exposed to toxicity induced by 
      linoleic acid hydroperoxide (LOOH). In the case where cells were incubated in 
      medium containing both LOOH and catechins, (+)-catechin (C) was effective in 
      suppressing of LOOH-induced cytotoxicity, but (-)-epicatechin (EC), 
      (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and 
      (-)-epigallocatechin gallate (EGCG) had no effect. EGCG monoglucoside (EGCG-G1) 
      and EGCG diglucoside (EGCG-G2), apophilic derivatives of EGCG, show a protective 
      effect on LOOH-induced cytotoxicity when present at the time of treatment with 
      LOOH. On the other hand, when cells were incubated with catechins for 24 h before 
      treatment with LOOH there was no protection against the oxidative damage by LOOH. 
      Furthermore, the interaction between catechins and alpha-tocopherol was examined 
      under these culture conditions. C showed a synergistic effect with 
      alpha-tocopherol in protecting against LOOH-induced damage. These results suggest 
      that catechins interact with LOOH present in the medium or near the surface of 
      membranes, but not with LOOH incorporated into cellular membranes and that 
      catechins are able to interact with alpha-tocopherol to provide synergistic 
      protection against the cytotoxicity of LOOH.
FAU - Kaneko, T
AU  - Kaneko T
AD  - Laboratory of Biochemistry and Isotopes, Tokyo Metropolitan Institute of 
      Gerontology, Japan. kaneko@center.tmig.or.jp
FAU - Matsuo, M
AU  - Matsuo M
FAU - Baba, N
AU  - Baba N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - Chem Biol Interact
JT  - Chemico-biological interactions
JID - 0227276
RN  - 0 (Flavonoids)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Linoleic Acids)
RN  - 0 (Lipid Peroxides)
RN  - 1406-18-4 (Vitamin E)
RN  - 25657-09-4 (linoleic acid hydroperoxide)
RN  - 8R1V1STN48 (Catechin)
RN  - 92587OVD8Z (epicatechin gallate)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - HEJ6575V1X (gallocatechol)
SB  - IM
MH  - Catechin/analogs & derivatives/*pharmacology
MH  - Cells, Cultured
MH  - Drug Interactions
MH  - Endothelium, Vascular/*drug effects
MH  - Flavonoids/pharmacology
MH  - Free Radical Scavengers/*pharmacology
MH  - Humans
MH  - Linoleic Acids/*antagonists & inhibitors/toxicity
MH  - Lipid Peroxides/*antagonists & inhibitors/toxicity
MH  - Oxidative Stress/drug effects
MH  - Structure-Activity Relationship
MH  - Umbilical Veins/*drug effects
MH  - Vitamin E/pharmacology
EDAT- 1998/09/23 00:00
MHDA- 1998/09/23 00:01
CRDT- 1998/09/23 00:00
PHST- 1998/09/23 00:00 [pubmed]
PHST- 1998/09/23 00:01 [medline]
PHST- 1998/09/23 00:00 [entrez]
AID - S0009-2797(98)00055-6 [pii]
AID - 10.1016/s0009-2797(98)00055-6 [doi]
PST - ppublish
SO  - Chem Biol Interact. 1998 Jul 3;114(1-2):109-19. doi: 
      10.1016/s0009-2797(98)00055-6.