PMID- 9745664
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20061115
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 29
IP  - 4
DP  - 1998 Aug
TI  - Comparative mapping of bovine chromosome 13 by fluorescence in situ 
      hybridization.
PG  - 265-72
AB  - We present chromosomal fluorescence in situ hybridization (FISH) results that 
      both extend the HSA20/BTA13 comparative map as well as cytogenetically anchor two 
      microsatellite markers. A bovine bacterial artificial chromosome (BAC) library 
      was screened for conserved genes (type 1 loci) previously assigned to HSA10 or 
      HSA20 and BTA13, and for microsatellites selected from two published BTA13 
      linkage maps. Clones from six out of nine comparative loci and both 
      microsatellites were found represented in the BAC library. These BAC clones were 
      used as probes in single colour FISH to determine the chromosome band position of 
      each locus. As predicted by the human/bovine comparative map, all type 1 loci 
      mapped to BTA13. Because single colour FISH analysis revealed that the loci were 
      clustered within the distal half of BTA13, dual colour FISH was used to confirm 
      the locus order. Established order was 
      centromere-PRNP-(SOD1L/AVP/OXT)-(BL42/GNAS1)- HCK-CSSM30. The findings confirm 
      the presence of a conserved HSA20 homologous synteny group on BTA13 distal of a 
      HSA10 homologous segment.
FAU - Schlapfer, J
AU  - Schlapfer J
AD  - Department of Veterinary Pathobiology, Crop Sciences, Texas A&M University, 
      College Station 77843, USA.
FAU - Gallagher, D S Jr
AU  - Gallagher DS Jr
FAU - Burzlaff, J D
AU  - Burzlaff JD
FAU - Womack, J E
AU  - Womack JE
FAU - Stelly, D M
AU  - Stelly DM
FAU - Taylor, J F
AU  - Taylor JF
FAU - Davis, S K
AU  - Davis SK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Cattle/*genetics
MH  - Chromosome Banding/veterinary
MH  - Chromosome Mapping/veterinary
MH  - Chromosomes, Bacterial
MH  - DNA Primers
MH  - Gene Library
MH  - In Situ Hybridization, Fluorescence/veterinary
EDAT- 1998/09/24 02:03
MHDA- 2001/03/28 10:01
CRDT- 1998/09/24 02:03
PHST- 1998/09/24 02:03 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/09/24 02:03 [entrez]
PST - ppublish
SO  - Anim Genet. 1998 Aug;29(4):265-72.