PMID- 9748498
OWN - NLM
STAT- MEDLINE
DCOM- 19981020
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 418
IP  - 1
DP  - 1998 Sep 25
TI  - The influence of DNA ploidy of a human tumor cell line on the frequencies of 
      micronuclei or chromosome aberrations after irradiation.
PG  - 49-57
AB  - To develop methods for assessing the intrinsic cellular radiosensitivity, it is 
      important to evaluate the relationship between DNA ploidy of cells and 
      frequencies of micronuclei (MN) or chromosome aberrations after irradiation. From 
      the original human fibrosarcoma cell line HT-1080, we isolated two clones which 
      have different chromosome ploidy: clone 5 is pseudodiploid and clone 1 is 
      heteroploid. We examined the radiosensitivity of the two clones using a 
      clonogenic cell survival assay and a cytokinesis-block MN assay, and by scoring 
      chromosome aberrations using the premature chromosome condensation (PCC) method 
      combined with a fluorescence in situ hybridization (FISH) procedure immediately 
      and at 24 h after irradiation. The MN frequency increased according to the 
      irradiation dose in both clones. The MN frequency of clone 1 was significantly 
      higher than that of clone 5 regardless of whether the assay was performed 
      immediately or 24 h after irradiation. However, when the numbers of MN were 
      normalized by the DNA index of each clone, a significant difference in the 
      frequency of MN was not observed. In the PCC and FISH studies, there was a linear 
      relationship between the radiation dose and the initial breaks of chromosome 4, 
      but the breaks of clone 1 were much more frequent than those of clone 5. 
      Twenty-four h after irradiation, the chromosome 4 breaks of clone 1 were observed 
      much more frequently than those of clone 5 at the same radiation dose. When the 
      numbers of chromosome 4 breaks were normalized by the number of chromosome 4 in 
      each clone without radiation, no such difference in the number of breaks was 
      observed. These findings demonstrated that the DNA content or chromosome ploidy 
      influenced the induction of the MN or chromosome aberrations in HT-1080 cells 
      after irradiation.
CI  - Copyright 1998 Elsevier Science B.V.
FAU - Takagi, T
AU  - Takagi T
AD  - Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, 
      Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
FAU - Sasai, K
AU  - Sasai K
FAU - Shibamoto, Y
AU  - Shibamoto Y
FAU - Akagi, K
AU  - Akagi K
FAU - Oya, N
AU  - Oya N
FAU - Shibata, T
AU  - Shibata T
FAU - Kim, J
AU  - Kim J
FAU - Hiraoka, M
AU  - Hiraoka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Cell Survival/radiation effects
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/genetics/*radiation effects
MH  - HeLa Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Micronucleus Tests
MH  - *Ploidies
MH  - Tumor Cells, Cultured
EDAT- 1998/09/28 00:00
MHDA- 1998/09/28 00:01
CRDT- 1998/09/28 00:00
PHST- 1998/09/28 00:00 [pubmed]
PHST- 1998/09/28 00:01 [medline]
PHST- 1998/09/28 00:00 [entrez]
AID - S1383-5718(98)00115-6 [pii]
AID - 10.1016/s1383-5718(98)00115-6 [doi]
PST - ppublish
SO  - Mutat Res. 1998 Sep 25;418(1):49-57. doi: 10.1016/s1383-5718(98)00115-6.