PMID- 9749314
OWN - NLM
STAT- MEDLINE
DCOM- 19981027
LR  - 20201209
IS  - 0132-3423 (Print)
IS  - 0132-3423 (Linking)
VI  - 24
IP  - 7
DP  - 1998 Jul
TI  - [Production of recombinant human keratinocyte growth factor in Escherichia coli 
      cells].
PG  - 523-9
AB  - Here we describe the synthesis of the gene encoding human keratinocyte growth 
      factor (hKGF) and the construction of vectors for cytoplasmic production of hKGF 
      in Escherichia coli cells. The level of recombinant protein expression was 1-1.5% 
      of the total cell protein. hKGF was purified to homogeneity by three-step 
      ion-exchange and affinity chromatography. The protein is biologically active: it 
      stimulates dose-dependent protein synthesis in cultured human epidermal 
      keratinocytes.
FAU - Ptitsyn, L R
AU  - Ptitsyn LR
AD  - State Research Institute Genetika, Moscow, Russia.
FAU - Al'tman, I B
AU  - Al'tman IB
FAU - Gurov, M V
AU  - Gurov MV
FAU - Vasil'ev, A V
AU  - Vasil'ev AV
FAU - Voroteliak, E A
AU  - Voroteliak EA
FAU - Terskikh, V V
AU  - Terskikh VV
LA  - rus
PT  - Journal Article
TT  - Produktsiia rekombinantnogo faktora rosta keratinotsitov cheloveka v kletkakh 
      Escherichia coli.
PL  - Russia (Federation)
TA  - Bioorg Khim
JT  - Bioorganicheskaia khimiia
JID - 7804941
RN  - 0 (FGF7 protein, human)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Growth Substances)
RN  - 0 (Recombinant Proteins)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chromatography, Affinity
MH  - Chromatography, Ion Exchange
MH  - Escherichia coli/genetics/*metabolism
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - Gene Expression
MH  - Genetic Vectors
MH  - Growth Substances/*biosynthesis/genetics/pharmacology
MH  - Humans
MH  - Keratinocytes/cytology/drug effects/*metabolism
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/biosynthesis/genetics/pharmacology
EDAT- 1998/09/28 00:00
MHDA- 1998/09/28 00:01
CRDT- 1998/09/28 00:00
PHST- 1998/09/28 00:00 [pubmed]
PHST- 1998/09/28 00:01 [medline]
PHST- 1998/09/28 00:00 [entrez]
PST - ppublish
SO  - Bioorg Khim. 1998 Jul;24(7):523-9.