PMID- 9749715
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20190815
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 10
IP  - 2
DP  - 1998 Feb
TI  - Development and characterization of antibodies directed against the mouse D4 
      dopamine receptor.
PG  - 529-37
AB  - Polyclonal antibodies against the mouse D4 dopamine receptor have been developed 
      in order to investigate the anatomical localization of this receptor in the mouse 
      brain. Two antibodies were generated against specific peptides corresponding to 
      predicted extracellular and intracellular regions of the D4 protein. Specificity 
      of these antibodies was demonstrated on human embryonic kidney 293 (HEK 293) 
      cells transfected with different dopamine receptor subtypes; immunoreactivity was 
      detected only in cells transfected with the mouse D4 dopamine receptor cDNA. 
      Following in vitro transcription/translation of the mouse D4 cDNA, a single 
      protein band of 36 kDa was selectively immunoprecipitated with the anti-D4 
      antibodies. The antibodies also detected a single protein of 36 kDa in Western 
      blot of HEK 293 cells transiently transfected with the mouse D4 receptor. These 
      antibodies were able to detect the D4 receptor in several regions of the mouse 
      brain. In the regions examined, D4 immunoreactivity was found in neurones located 
      in layers II-VI of the frontal and piriform cortices, with the highest 
      concentration in layer II; in scattered neurones in the caudate putamen and in 
      larger neurones in the globus pallidus. In all experiments, both antibodies 
      exhibit the same specificity, and all immunoreactivity could be abolished by 
      preincubation with the corresponding peptide antigen.
FAU - Mauger, C
AU  - Mauger C
AD  - Hoffmann-La Roche Ltd, CNS preclinical research, Basel, Switzerland.
FAU - Sivan, B
AU  - Sivan B
FAU - Brockhaus, M
AU  - Brockhaus M
FAU - Fuchs, S
AU  - Fuchs S
FAU - Civelli, O
AU  - Civelli O
FAU - Monsma, F Jr
AU  - Monsma F Jr
LA  - eng
PT  - Journal Article
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies, Blocking)
RN  - 0 (DRD4 protein, human)
RN  - 0 (Dopamine D2 Receptor Antagonists)
RN  - 0 (Drd4 protein, mouse)
RN  - 0 (Receptors, Dopamine D2)
RN  - 137750-34-6 (Receptors, Dopamine D4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Blocking/chemistry/immunology/*pharmacology
MH  - Blotting, Western
MH  - Cell Line
MH  - *Dopamine D2 Receptor Antagonists
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Mice
MH  - Molecular Conformation
MH  - Molecular Sequence Data
MH  - Precipitin Tests
MH  - Receptors, Dopamine D2/immunology
MH  - Receptors, Dopamine D4
MH  - Tissue Fixation
EDAT- 1998/09/28 15:36
MHDA- 2001/03/28 10:01
CRDT- 1998/09/28 15:36
PHST- 1998/09/28 15:36 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/09/28 15:36 [entrez]
AID - 10.1046/j.1460-9568.1998.00056.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 1998 Feb;10(2):529-37. doi: 10.1046/j.1460-9568.1998.00056.x.