PMID- 9750123 OWN - NLM STAT- MEDLINE DCOM- 19980924 LR - 20200724 IS - 0099-2240 (Print) IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) VI - 64 IP - 8 DP - 1998 Aug TI - Strain IMB-1, a novel bacterium for the removal of methyl bromide in fumigated agricultural soils. PG - 2899-905 AB - A facultatively methylotrophic bacterium, strain IMB-1, that has been isolated from agricultural soil grows on methyl bromide (MeBr), methyl iodide, methyl chloride, and methylated amines, as well as on glucose, pyruvate, or acetate. Phylogenetic analysis of its 16S rRNA gene sequence indicates that strain IMB-1 classes in the alpha subgroup of the class Proteobacteria and is closely related to members of the genus Rhizobium. The ability of strain IMB-1 to oxidize MeBr to CO2 is constitutive in cells regardless of the growth substrate. Addition of cell suspensions of strain IMB-1 to soils greatly accelerates the oxidation of MeBr, as does pretreatment of soils with low concentrations of methyl iodide. These results suggest that soil treatment strategies can be devised whereby bacteria can effectively consume MeBr during field fumigations, which would diminish or eliminate the outward flux of MeBr to the atmosphere. FAU - Hancock, T L AU - Hancock TL AD - U.S. Geological Survey, Menlo Park, California 94025, USA. FAU - Costello, A M AU - Costello AM FAU - Lidstrom, M E AU - Lidstrom ME FAU - Oremland, R S AU - Oremland RS LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 RN - 0 (Culture Media) RN - 0 (Hydrocarbons, Brominated) RN - 0 (Pesticides) RN - 9V42E1Z7B6 (methyl bromide) SB - IM MH - Biodegradation, Environmental MH - Culture Media MH - *Fumigation MH - Gram-Negative Bacteria/classification/growth & development/*isolation & purification/*metabolism MH - Hydrocarbons, Brominated/*metabolism MH - Oxidation-Reduction MH - Pesticides/*metabolism MH - Phylogeny MH - *Soil Microbiology PMC - PMC106790 EDAT- 1998/08/04 00:00 MHDA- 1998/08/04 00:01 PMCR- 1998/08/01 CRDT- 1998/08/04 00:00 PHST- 1998/08/04 00:00 [pubmed] PHST- 1998/08/04 00:01 [medline] PHST- 1998/08/04 00:00 [entrez] PHST- 1998/08/01 00:00 [pmc-release] AID - 0284 [pii] AID - 10.1128/AEM.64.8.2899-2905.1998 [doi] PST - ppublish SO - Appl Environ Microbiol. 1998 Aug;64(8):2899-905. doi: 10.1128/AEM.64.8.2899-2905.1998.