PMID- 9753202
OWN - NLM
STAT- MEDLINE
DCOM- 19981222
LR  - 20220616
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 53
IP  - 6
DP  - 1998 Sep 15
TI  - TNF-alpha and TGF-beta act synergistically to kill Schwann cells.
PG  - 747-56
AB  - Interactions between cytokines and Schwann cells (SC) are important in 
      development, repair, and disorders of the peripheral nervous system (PNS). Tumor 
      necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta) 
      are two prominent cytokines which may be involved in these processes and their 
      gene products are upregulated in some experimental neuropathies. This study 
      focuses on the in vitro effects of these cytokines, both singly and in 
      combination, on cultured SC. Expression of both Type I and Type II TNF-alpha 
      receptors was demonstrated on the SC surface by immunocytochemistry. Treatment of 
      SC with a combination of TNF-alpha plus TGF-beta causes significant detachment 
      and cell death while treatment with each cytokine alone is not significantly 
      cytotoxic. When compared with control cultures, SC treated with the combination 
      of cytokines exhibit an increase in the number of cells with condensed nuclei and 
      evidence of DNA fragmentation, characteristics consistent with cells undergoing 
      programmed cell death. Thus, TNF-alpha plus TGF-beta induce SC loss of adhesion 
      which is predominantly due to cell death. Apoptotic mechanisms are likely to 
      contribute to some extent to this cell death. These findings provide in vitro 
      evidence to support the hypothesis that cytokines can directly damage SC in PNS 
      disorders.
FAU - Skoff, A M
AU  - Skoff AM
AD  - Department of Neurology, Wayne State University School of Medicine, 6E-University 
      Health Center, Detroit, Michigan 48201, USA.
FAU - Lisak, R P
AU  - Lisak RP
FAU - Bealmear, B
AU  - Bealmear B
FAU - Benjamins, J A
AU  - Benjamins JA
LA  - eng
GR  - NS31287/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Apoptosis/physiology
MH  - Cell Adhesion/drug effects
MH  - Cell Survival/drug effects
MH  - Drug Synergism
MH  - Isomerism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Tumor Necrosis Factor/metabolism
MH  - Recombinant Proteins
MH  - Schwann Cells/cytology/*drug effects/metabolism/physiology
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism/*pharmacology
EDAT- 1998/09/30 02:03
MHDA- 2000/06/20 09:00
CRDT- 1998/09/30 02:03
PHST- 1998/09/30 02:03 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/09/30 02:03 [entrez]
AID - 10.1002/(SICI)1097-4547(19980915)53:6<747::AID-JNR12>3.0.CO;2-V [pii]
AID - 10.1002/(SICI)1097-4547(19980915)53:6<747::AID-JNR12>3.0.CO;2-V [doi]
PST - ppublish
SO  - J Neurosci Res. 1998 Sep 15;53(6):747-56. doi: 
      10.1002/(SICI)1097-4547(19980915)53:6<747::AID-JNR12>3.0.CO;2-V.