PMID- 9754956
OWN - NLM
STAT- MEDLINE
DCOM- 19990602
LR  - 20190812
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 96
IP  - 3
DP  - 1998 Sep
TI  - Murine cytomegalovirus induces apoptosis in non-infected cells of the developing 
      mouse brain and blocks apoptosis in primary neuronal culture.
PG  - 239-47
AB  - Cytomegalovirus (CMV) is the most common cause of congenital infection, resulting 
      in birth defects such as microcephaly. In this study, we found that apoptosis is 
      induced in the developing mouse brain infected with murine cytomegalovirus (MCMV) 
      in an association with neuronal cell loss. With the combination of the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique 
      and immunohistochemical staining, 3.8% of the TUNEL-positive cells were 
      double-stained with the antibody to neuron-specific enolase, while none of the 
      TUNEL-positive cells were stained with antibodies to the immediate early and 
      early viral antigens of MCMV. Furthermore, distribution pattern of the 
      TUNEL-positive cells was different from that of viral DNA-positive cells detected 
      by the in situ DNA-DNA hybridization. More than 30% of the TUNEL-positive cells 
      were double-stained with the F4/80 antibody specific for microglia/macrophages, 
      which were sometimes swollen, presumably the consequence of engulfment of the 
      neuronal apoptotic cells. In the primary neuronal cultures, MCMV infection 
      inhibited the induction of apoptosis either by serum deprivation or by glutamate 
      treatment. It was also confirmed by the double-staining method that apoptosis was 
      not induced in the viral-infected neuronal cultures. These results suggest that 
      MCMV infection induces apoptosis in non-infected neuronal cells, presumably by 
      indirect mechanisms, and that apoptotic cells are engulfed by 
      microglia/macrophages. The induction and blocking of neuronal apoptosis by viral 
      infection may be important for morphological and functional brain disorders in 
      the congenital CMV infection.
FAU - Kosugi, I
AU  - Kosugi I
AD  - Second Department of Pathology, Hamamatsu University School of Medicine, Japan.
FAU - Shinmura, Y
AU  - Shinmura Y
FAU - Li, R Y
AU  - Li RY
FAU - Aiba-Masago, S
AU  - Aiba-Masago S
FAU - Baba, S
AU  - Baba S
FAU - Miura, K
AU  - Miura K
FAU - Tsutsui, Y
AU  - Tsutsui Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
SB  - IM
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Brain/*growth & development/*pathology/virology
MH  - Cells, Cultured
MH  - Cytomegalovirus/*pathogenicity
MH  - Cytomegalovirus Infections
MH  - Embryo, Mammalian
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Microscopy, Electron
MH  - Neurons/chemistry/*cytology/*virology
EDAT- 1998/10/01 00:00
MHDA- 1998/10/01 00:01
CRDT- 1998/10/01 00:00
PHST- 1998/10/01 00:00 [pubmed]
PHST- 1998/10/01 00:01 [medline]
PHST- 1998/10/01 00:00 [entrez]
AID - 10.1007/s004010050890 [doi]
PST - ppublish
SO  - Acta Neuropathol. 1998 Sep;96(3):239-47. doi: 10.1007/s004010050890.