PMID- 9755123
OWN - NLM
STAT- MEDLINE
DCOM- 19981119
LR  - 20190301
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 275
IP  - 4
DP  - 1998 Oct
TI  - Inducible transcriptional activity of bcn-1 element from laminin gamma1-chain 
      gene promoter in renal and nonrenal cells.
PG  - F518-26
LID - 10.1152/ajprenal.1998.275.4.F518 [doi]
AB  - Laminin is a major component of the extracellular matrix whose expression is 
      regulated by growth factors. The laminin gamma1-chain promoter contains a newly 
      identified transcriptional element denoted bcn-1 that is both active and 
      inducible in mesangial cells. In this study, we explored activation of the bcn-1 
      element in other renal and nonrenal cells. Treatment of rat glomerular epithelial 
      cells (GEC) with phorbol 12-myristate 13-acetate (PMA) increased activity of the 
      bcn-1 transcriptional element, within the context of the native laminin 
      gamma1-chain promoter or when cloned upstream of a heterologous promoter. 
      Treatment of GEC with PMA induced nuclear DNA-binding activity, BCN-1, which was 
      recognized by the bcn-1 motif in a gel shift assay. These results provide 
      evidence that the bcn-1 motif and its cognate BCN-1 factor(s) may regulate 
      transcription of the laminin gamma1-chain in GEC. The bcn-1 element and its 
      cognate BCN-1 DNA-binding activity were also inducible in monkey kidney COS-7 and 
      in human T cell Jurkat lines. SDS-PAGE of in situ ultraviolet cross-linked 
      nucleoproteins from GEC, COS, and Jurkat cells revealed one major 110-115 kDa 
      adduct in all three cell lines. These results demonstrate that the bcn-1 element 
      is active in renal and nonrenal cells from different mammalian species where the 
      same protein contributes to the inducible BCN-1 DNA-binding activity.
FAU - Suzuki, H
AU  - Suzuki H
AD  - Department of Medicine, University of Washington, Seattle, Washington 98195, USA.
FAU - Denisenko, O N
AU  - Denisenko ON
FAU - Suzuki, Y
AU  - Suzuki Y
FAU - Schullery, D S
AU  - Schullery DS
FAU - Bomsztyk, K
AU  - Bomsztyk K
LA  - eng
GR  - DK-45978/DK/NIDDK NIH HHS/United States
GR  - GM-45134/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Laminin)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (laminin gamma 1)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Binding Sites
MH  - COS Cells
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA-Binding Proteins/*metabolism
MH  - Epithelial Cells/drug effects/metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - Kidney Glomerulus/drug effects/*metabolism
MH  - Laminin/biosynthesis/*genetics
MH  - Nuclear Proteins/*metabolism
MH  - *Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - *Transcription, Genetic
MH  - Transfection
EDAT- 1998/10/01 00:00
MHDA- 1998/10/01 00:01
CRDT- 1998/10/01 00:00
PHST- 1998/10/01 00:00 [pubmed]
PHST- 1998/10/01 00:01 [medline]
PHST- 1998/10/01 00:00 [entrez]
AID - 10.1152/ajprenal.1998.275.4.F518 [doi]
PST - ppublish
SO  - Am J Physiol. 1998 Oct;275(4):F518-26. doi: 10.1152/ajprenal.1998.275.4.F518.