PMID- 9756294
OWN - NLM
STAT- MEDLINE
DCOM- 19981217
LR  - 20190512
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 13
IP  - 8
DP  - 1998 Aug
TI  - Leukocytes in normal-cycling human ovaries: immunohistochemical distribution and 
      characterization.
PG  - 2186-91
AB  - We investigated, using an image analysis system, the immunohistochemical 
      localization of leukocyte subpopulations and human leukocyte antigen (HLA)-DR in 
      30 normal-cycling human ovaries in order to better understand local immunological 
      events in human ovaries. All subtypes of T lymphocytes examined (CD3+, CD4+ and 
      CD8+ cells) were sporadically observed in the stroma and theca layers of 
      follicles throughout the menstrual cycle (ranging from 4.32 to 6.25 cells/10(-7) 
      m2, 1.67 to 3.33 cells/10(-7) m2 and 2.33 to 3.44 cells/10(-7) m2, respectively 
      for the three subtypes), and subsequently, increased in number in atretic 
      follicles (78.70 +/- 6.90, 31.13 +/- 2.54 and 43.31 +/- 3.35). After ovulation, 
      the number of T lymphocytes was markedly low in the early and mid corpus luteum 
      (13.88 +/- 1.62, 4.18 +/- 0.50 and 6.53 +/- 0.45). The number increased in the 
      late corpus luteum, and was highest in the late degenerating corpus luteum 
      (255.67 +/- 27.10, 102.12 +/- 7.80 and 137.34 +/- 12.50). HLA-DR was sporadically 
      positive in fibroblasts in the stroma and theca layers of follicles (means ranged 
      from 1.25 to 1.82 cells/10(-7) m2), and increased in atretic follicles (24.68 +/- 
      2.25). HLA-DR+ cells were markedly low in the early and mid corpus luteum (2.16 
      +/- 0.88), increased in the late corpus luteum, and reached a plateau in the late 
      degenerating corpus luteum (121.84 +/- 17.73). The great majority of these 
      increased HLA-DR+ cells were macrophages. Results of our study suggest that T 
      lymphocytes and/or macrophages play important roles in luteal regression and 
      follicular atresia in normal-cycling human ovaries.
FAU - Suzuki, T
AU  - Suzuki T
AD  - Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
FAU - Sasano, H
AU  - Sasano H
FAU - Takaya, R
AU  - Takaya R
FAU - Fukaya, T
AU  - Fukaya T
FAU - Yajima, A
AU  - Yajima A
FAU - Date, F
AU  - Date F
FAU - Nagura, H
AU  - Nagura H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adult
MH  - B-Lymphocytes/cytology/immunology
MH  - Female
MH  - Follicular Atresia/immunology
MH  - HLA-DR Antigens/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Leukocytes/*cytology/*immunology
MH  - Luteolysis/immunology
MH  - Macrophages/cytology/immunology
MH  - Menstrual Cycle/immunology
MH  - Middle Aged
MH  - Neutrophils/cytology/immunology
MH  - Ovary/*cytology/*immunology
MH  - T-Lymphocytes/cytology/immunology
EDAT- 1998/10/02 00:00
MHDA- 1998/10/02 00:01
CRDT- 1998/10/02 00:00
PHST- 1998/10/02 00:00 [pubmed]
PHST- 1998/10/02 00:01 [medline]
PHST- 1998/10/02 00:00 [entrez]
AID - 10.1093/humrep/13.8.2186 [doi]
PST - ppublish
SO  - Hum Reprod. 1998 Aug;13(8):2186-91. doi: 10.1093/humrep/13.8.2186.