PMID- 9756617
OWN - NLM
STAT- MEDLINE
DCOM- 19990121
LR  - 20190512
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 124
IP  - 4
DP  - 1998 Oct
TI  - Alpha 1,6-linked fucose affects the expression and stability of polysialic 
      acid-carrying glycoproteins in Chinese hamster ovary cells.
PG  - 726-37
AB  - To determine the effect of alpha1,6-linked fucose modification of N-glycans on 
      the expression of polysialic acids (PSAs), the expression of PSAs in a 
      fucose-lacking mutant of Chinese hamster ovary (CHO) cells, Lec13, was compared 
      with that in CHO K1 cells. PSA synthase activity in these cells and the antennary 
      structures of N-glycans associated with the neural adhesion molecule (NCAM), 
      which is a major PSA-carrying glycoprotein, did not differ between the two types 
      of cells. Metabolic labeling of cells with [3H]glucosamine for 48 h followed by 
      immunoprecipitation with anti-PSA monoclonal antibodies revealed that the amount 
      of labeled PSA-carrying glycoproteins obtained from Lec13 cells was 10-times less 
      than that from K1 cells, although the incorporation of [3H]glucosamine into total 
      extracts and NCAM was almost the same. In contrast, when cells were pulse labeled 
      with [35S]methionine followed by a 1 h chase, there was not such a great 
      difference in PSA-carrying protein synthesis between K1 and Lec13 cells. However, 
      during a prolonged chase period, PSA-carrying proteins rapidly decreased in Lec13 
      cells, whereas those in K1 cells did not change. The degradation of PSA-carrying 
      glycoproteins in Lec13 cells was partly prevented when the cells were grown in 
      fucose-containing medium. Therefore, fucose modification of core N-glycans may 
      affect the efficient expression of PSAs through the intracellular stability of 
      PSA-carrying glycoproteins.
FAU - Kojima, N
AU  - Kojima N
AD  - Molecular Glycobiology, Frontier Research Program, The Institute of Physical and 
      Chemical Research (RIKEN), Wako, Saitama, 351-0198, Japan.
FAU - Tachida, Y
AU  - Tachida Y
FAU - Tsuji, S
AU  - Tsuji S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Glycoproteins)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialic acid)
RN  - 10028-17-8 (Tritium)
RN  - 28RYY2IV3F (Fucose)
RN  - EC 2.4.99.- (CMP-N-acetylneuraminate-poly-alpha-2,8-sialosyl sialyltransferase)
RN  - EC 2.4.99.- (Sialyltransferases)
RN  - N08U5BOQ1K (Glucosamine)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - CHO Cells
MH  - Clone Cells
MH  - Cricetinae
MH  - Fucose/deficiency/*metabolism
MH  - Glucosamine/metabolism
MH  - Glycoproteins/biosynthesis/*metabolism
MH  - Kinetics
MH  - Neural Cell Adhesion Molecules/*biosynthesis
MH  - Sialic Acids/biosynthesis/*metabolism
MH  - Sialyltransferases/*metabolism
MH  - Tritium
EDAT- 1998/10/02 00:00
MHDA- 1998/10/02 00:01
CRDT- 1998/10/02 00:00
PHST- 1998/10/02 00:00 [pubmed]
PHST- 1998/10/02 00:01 [medline]
PHST- 1998/10/02 00:00 [entrez]
AID - 10.1093/oxfordjournals.jbchem.a022173 [doi]
PST - ppublish
SO  - J Biochem. 1998 Oct;124(4):726-37. doi: 10.1093/oxfordjournals.jbchem.a022173.