PMID- 9758040
OWN - NLM
STAT- MEDLINE
DCOM- 19981208
LR  - 20220310
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 19
IP  - 15
DP  - 1998 Aug
TI  - Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable 
      polymers.
PG  - 1405-12
AB  - Neonatal rat calvarial osteoblasts were cultured in 90% porous, 75:25 
      poly(DL-lactic-co-glycolic acid) (PLGA) foam scaffolds for up to 56 days to 
      examine the effects of the cell seeding density, scaffold pore size, and foam 
      thickness on the proliferation and function of the cells in this 
      three-dimensional environment. Osteoblasts were seeded at either 11.1 x 10(5) or 
      22.1 x 10(5) cells per cm2 onto PLGA scaffolds having pore sizes in the range of 
      150-300 or 500-710 microm with a thickness of either 1.9 or 3.2 mm. After 1 day 
      in culture, 75.6 and 68.6% of the seeded cells attached and proliferated on the 
      1.9 mm thick scaffolds of 150-300 microm pore size for the low and high seeding 
      densities, respectively. The number of osteoblasts continued to increase 
      throughout the study and eventually leveled off near 56 days, as indicated by a 
      quantitative DNA assay. Osteoblast/foam constructs with a low cell seeding 
      density achieved comparable DNA content and alkaline phosphatase (ALPase) 
      activity after 14 days, and mineralization results after 56 days to those with a 
      high cell seeding density. A maximum penetration depth of osseous tissue of 
      220+/-40 microm was reached after 56 days in the osteoblast/foam constructs of 
      150-300 microm pore size initially seeded with a high cell density. For 
      constructs of 500-710 microm pore size, the penetration depth was 190+/-40 microm 
      under the same conditions. Scaffold pore size and thickness did not significantly 
      affect the proliferation or function of osteoblasts as demonstrated by DNA 
      content, ALPase activity, and mineralized tissue formation. These data show that 
      comparable bone-like tissues can be engineered in vitro over a 56 day period 
      using different rat calvarial osteoblast seeding densities onto biodegradable 
      polymer scaffolds with pore sizes in the range of 150-710 microm. When compared 
      with the results of a previous study where similar polymer scaffolds were seeded 
      and cultured with marrow stromal cells, this study demonstrates that PLGA foams 
      are suitable substrates for osteoblast growth and differentiated function 
      independent of cell source.
FAU - Ishaug-Riley, S L
AU  - Ishaug-Riley SL
AD  - Institute of Biosciences and Bioengineering and Department of Chemical 
      Engineering, Rice University, Houston, TX 77005-1892, USA.
FAU - Crane-Kruger, G M
AU  - Crane-Kruger GM
FAU - Yaszemski, M J
AU  - Yaszemski MJ
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
GR  - R29-AR42639/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - *Biocompatible Materials
MH  - Bone Development/physiology
MH  - Bone Matrix/*cytology
MH  - Cell Adhesion/physiology
MH  - Cell Count
MH  - Cell Division/physiology
MH  - Cells, Cultured
MH  - *Lactic Acid
MH  - Osteoblasts/*cytology/enzymology
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - *Polymers
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NASA
OT  - NASA Discipline Cell Biology
OT  - Non-NASA Center
FIR - Mikos, A G
IR  - Mikos AG
IRAD- Rice U, Houston, TX
EDAT- 1998/10/03 00:00
MHDA- 1998/10/03 00:01
CRDT- 1998/10/03 00:00
PHST- 1998/10/03 00:00 [pubmed]
PHST- 1998/10/03 00:01 [medline]
PHST- 1998/10/03 00:00 [entrez]
AID - S0142-9612(98)00021-0 [pii]
AID - 10.1016/s0142-9612(98)00021-0 [doi]
PST - ppublish
SO  - Biomaterials. 1998 Aug;19(15):1405-12. doi: 10.1016/s0142-9612(98)00021-0.