PMID- 9758367
OWN - NLM
STAT- MEDLINE
DCOM- 19981202
LR  - 20151119
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 11
IP  - 9
DP  - 1998 Sep
TI  - Prognostic factors in gastric cancer.
PG  - 870-7
AB  - Molecular assays related to cell proliferation correlate with stage and/or 
      survival in a variety of tumors. We immunostained formalin-fixed, 
      paraffin-embedded tissue sections from 61 patients with gastric adenocarcinoma 
      (21 biopsy and 40 gastrectomy specimens) for cyclin A, cyclin B1, p34cdc2, p120, 
      MIB1, and proliferating cell nuclear antigen (PCNA) by automated methods. 
      HER-2/neu gene amplification was analyzed by automated fluorescence in situ 
      hybridization (FISH). Immununostains, FISH results, and pathologic stage were 
      compared with length of survival. Forty-three percent of the cases showed 
      amplification of HER-2/neu. Sixty-two percent of cases showed positive 
      immunostaining for cyclin A, 38% for cyclin B1, 31% for p34cdc2, 49% for p120, 
      69% for MIB1, and 33% for PCNA. On univariate analysis, pathologic stage (P = 
      .003) and HER-2/neu gene amplification (P < .001) correlated with length of 
      survival. Cyclin A, cyclin B1, p34cdc2, p120, MIB1, and PCNA did not correlate 
      with survival. On multivariate analysis, pathologic stage (P = .015) and 
      HER-2/neu gene amplification (P = .002) independently predicted survival. These 
      correlations were unrelated to tubular or signet ring cell histologic 
      characteristics or to location within the cardia or more distally. Pathologic 
      stage and HER-2/neu gene amplification by FISH were independent prognostic 
      factors in gastric cancer, but the various proliferation markers that we studied 
      did not correlate with survival.
FAU - Brien, T P
AU  - Brien TP
AD  - Department of Pathology and Laboratory Medicine, Albany Medical College and S. 
      Stratton Veterans Administration Medical Center, New York 12208, USA.
FAU - Depowski, P L
AU  - Depowski PL
FAU - Sheehan, C E
AU  - Sheehan CE
FAU - Ross, J S
AU  - Ross JS
FAU - McKenna, B J
AU  - McKenna BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multivariate Analysis
MH  - Prognosis
MH  - Receptor, ErbB-2/metabolism
MH  - Stomach Neoplasms/*diagnosis/metabolism/mortality/pathology
MH  - Survival Rate
EDAT- 1998/10/03 00:00
MHDA- 1998/10/03 00:01
CRDT- 1998/10/03 00:00
PHST- 1998/10/03 00:00 [pubmed]
PHST- 1998/10/03 00:01 [medline]
PHST- 1998/10/03 00:00 [entrez]
PST - ppublish
SO  - Mod Pathol. 1998 Sep;11(9):870-7.