PMID- 9758418
OWN - NLM
STAT- MEDLINE
DCOM- 19981223
LR  - 20191102
IS  - 0906-6705 (Print)
IS  - 0906-6705 (Linking)
VI  - 7
IP  - 4
DP  - 1998 Aug
TI  - Human tyrosinase related protein-1 (TRP-1) does not function as a DHICA oxidase 
      activity in contrast to murine TRP-1.
PG  - 198-204
AB  - Tyrosinase related protein-1 is a melanocyte specific protein and a member of the 
      tyrosinase gene family which also includes tyrosinase and TRP 2 (DOPAchrome 
      tautomerase). In murine melanocytes, TRP-1 functions as a 
      5,6-dihydroxyindole-2-carboxylic acid [DHICA] oxidase during the biosynthetic 
      conversion of tyrosine to eumelanin and mutations affecting TRP-1 result in the 
      synthesis of brown rather than black pelage coloration. In this study, we 
      examined the putative DHICA oxidase activity of TRP-1 in human melanocytes using 
      several approaches. We first utilized a line of cultured melanocytes established 
      from a patient with a form of oculocutaneous albinism completely lacking 
      expression of TRP-1 (OCA3). This line of melanocytes endogenously exhibited the 
      same amount of DHICA oxidase activity as control melanocytes expressing TRP-1. In 
      other experiments, cultured human fibroblasts were transfected with a cDNA for 
      TRP-1, in either the sense or antisense direction, or with the retroviral vector 
      alone. TRP-1 expression was induced in fibroblasts transfected with the TRP-1 
      cDNA in the sense direction only. Although TRP-1 was expressed by 
      sense-transfected cells, there was no significant DHICA oxidase activity above 
      controls. These results demonstrate that human TRP-1 does not use DHICA as a 
      substrate for oxidation.
FAU - Boissy, R E
AU  - Boissy RE
AD  - Department of Dermatology, University of Cincinnati College of Medicine, OH, USA. 
      boissyre@email.uc.edu
FAU - Sakai, C
AU  - Sakai C
FAU - Zhao, H
AU  - Zhao H
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Hearing, V J
AU  - Hearing VJ
LA  - eng
GR  - AR43368/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteins)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (TYRP1 protein, human)
RN  - EC 1.14.18.- (Tyrp1 protein, mouse)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Melanocytes/*metabolism
MH  - *Membrane Glycoproteins
MH  - Mice
MH  - Oxidoreductases/chemistry/*metabolism
MH  - Proteins/chemistry/*metabolism
MH  - Species Specificity
MH  - Transfection
EDAT- 1998/10/03 00:00
MHDA- 1998/10/03 00:01
CRDT- 1998/10/03 00:00
PHST- 1998/10/03 00:00 [pubmed]
PHST- 1998/10/03 00:01 [medline]
PHST- 1998/10/03 00:00 [entrez]
AID - 10.1111/j.1600-0625.1998.tb00324.x [doi]
PST - ppublish
SO  - Exp Dermatol. 1998 Aug;7(4):198-204. doi: 10.1111/j.1600-0625.1998.tb00324.x.