PMID- 9759917
OWN - NLM
STAT- MEDLINE
DCOM- 19981211
LR  - 20190831
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 33
IP  - 3
DP  - 1998 Sep
TI  - L-arginine uptake in rat cerebral mitochondria.
PG  - 233-6
AB  - The kinetics of L-[14C]arginine (L-[14C]Arg) uptake and the effects of potential 
      competitors on the uptake were analysed in nonsynaptic mitochondria isolated from 
      rat cerebral hemispheres. Analysis of uptake kinetics revealed a high affinity 
      component with a mean Km = 0.08 mM, and Vmax = 1.89 nmoles/min/mg, and a very low 
      affinity component probably manifesting diffusion. The uptake of 25 mM L-Arg was 
      strongly inhibited by a 20-fold excess of L-lysine (L-Lys) and L-ornithine 
      (L-Orn), but not by D-Arg nor any neutral amino acid, which resembles the 
      characteristics of the gamma+ transport system operating in the nerve- and glia 
      cell-, and synaptic plasma membranes. Also in consistance with the other gamma+ 
      systems, L-Arg uptake to mitochondria was inhibited by a nitric oxide synthase 
      (NOS) inhibitor L-N-monomethyl arginine (L-NMMA), but not by another NOS 
      inhibitor NG-nitro-L-arginine (L-NNA). However, the uptake was very little 
      affected by 20-fold excess of L-histidine (L-His), L-glutamate (L-Glu) or 
      L-glutamine (L-Gln), which is in contrast to the nonmitochondrial systems. The 
      uptake was only marginally influenced by cytoplasmic L-Arg metabolites: ammonia, 
      creatine, putrescine, or the mitochondrial L-Arg decarboxylation product, 
      agmatine.
FAU - Dolinska, M
AU  - Dolinska M
AD  - Department of Neurotoxicology, Medical Research Centre, Polish Academy of 
      Sciences, Warszawa.
FAU - Albrecht, J
AU  - Albrecht J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Enzyme Inhibitors)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 94ZLA3W45F (Arginine)
RN  - E524N2IXA3 (Ornithine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - K3Z4F929H6 (Lysine)
SB  - IM
MH  - Animals
MH  - Arginine/*metabolism
MH  - Biological Transport/drug effects
MH  - Brain/*ultrastructure
MH  - Carbon Radioisotopes
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Kinetics
MH  - Lysine/pharmacology
MH  - Mitochondria/*metabolism
MH  - Nitric Oxide Synthase/antagonists & inhibitors
MH  - Ornithine/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - omega-N-Methylarginine/pharmacology
EDAT- 1998/10/06 00:00
MHDA- 1998/10/06 00:01
CRDT- 1998/10/06 00:00
PHST- 1998/10/06 00:00 [pubmed]
PHST- 1998/10/06 00:01 [medline]
PHST- 1998/10/06 00:00 [entrez]
AID - S0197-0186(98)00020-5 [pii]
AID - 10.1016/s0197-0186(98)00020-5 [doi]
PST - ppublish
SO  - Neurochem Int. 1998 Sep;33(3):233-6. doi: 10.1016/s0197-0186(98)00020-5.