PMID- 9760154
OWN - NLM
STAT- MEDLINE
DCOM- 19981016
LR  - 20190921
IS  - 0939-5555 (Print)
IS  - 0939-5555 (Linking)
VI  - 77
IP  - 1-2
DP  - 1998 Jul-Aug
TI  - Molecular detection of a late-appearing BCR-ABL gene in a child with T-cell acute 
      lymphoblastic leukemia.
PG  - 55-9
AB  - Approximately 2-5% of children with newly diagnosed acute lymphoblastic leukemia 
      (ALL) have a Philadelphia (Ph) chromosome detectable on cytogenetic analysis, 
      which is associated with a poor prognosis. In rare ALL cases the Ph chromosome 
      may appear in leukemic cells during the course of the disease. We report here the 
      case of a 5.5-year-old male patient with T-ALL who was found to have the b2a2 
      BCR-ABL mRNA transcript by reverse transcriptase-polymerase chain reaction 
      (RT-PCR) at first marrow relapse. At the time of initial diagnosis, no BCR-ABL 
      transcripts had been detected by PCR in the patient's blood and marrow samples. 
      Further studies were performed using a competitive PCR titration assay and the 
      fluorescence in situ hybridization (FISH) method to monitor the leukemic clone. 
      Progression of the disease was associated with a higher BCR-ABL transcript level 
      and an increasing proportion of BCR-ABL-positive cells. Metaphase FISH analysis 
      identified the presence of the BCR-ABL fusion gene on a normal chromosome 22. 
      This study shows that a late-appearing Ph translocation in ALL may be 
      cytogenetically invisible. Quantitative RT-PCR and FISH techniques are 
      appropriate and efficient methods for detecting these rare ALL variants 
      expressing the BCR-ABL fusion gene and for estimating the level of residual 
      disease following treatment.
FAU - Tchirkov, A
AU  - Tchirkov A
AD  - Laboratoire de Cytogenetique Medicale, Faculte de Medecine, Clermont-Ferrand, 
      France.
FAU - Bons, J M
AU  - Bons JM
FAU - Chassagne, J
AU  - Chassagne J
FAU - Schoepfer, C
AU  - Schoepfer C
FAU - Kanold, J
AU  - Kanold J
FAU - Briancon, G
AU  - Briancon G
FAU - Giollant, M
AU  - Giollant M
FAU - Malet, P
AU  - Malet P
FAU - Demeocq, F
AU  - Demeocq F
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Child
MH  - Cytogenetics
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - *Genes, Neoplasm
MH  - Humans
MH  - Karyotyping
MH  - Leukemia-Lymphoma, Adult T-Cell/*genetics
MH  - Male
EDAT- 1998/10/06 00:00
MHDA- 1998/10/06 00:01
CRDT- 1998/10/06 00:00
PHST- 1998/10/06 00:00 [pubmed]
PHST- 1998/10/06 00:01 [medline]
PHST- 1998/10/06 00:00 [entrez]
AID - 10.1007/s002770050412 [doi]
PST - ppublish
SO  - Ann Hematol. 1998 Jul-Aug;77(1-2):55-9. doi: 10.1007/s002770050412.