PMID- 9760868
OWN - NLM
STAT- MEDLINE
DCOM- 19981013
LR  - 20190607
IS  - 0018-0661 (Print)
IS  - 0018-0661 (Linking)
VI  - 128
IP  - 3
DP  - 1998
TI  - Physical mapping and activity of ribosomal RNA genes in mussel Mytilus 
      galloprovincialis.
PG  - 189-94
AB  - In bivalve molluscs, NOR analysis was carried out by silver staining, and 
      extensive intra- and interindividual differences in the apparent number of NORs 
      were reported. In this work, we determine the physical mapping of 18S and 28S 
      ribosomal genes of the mussel M. galloprovincialis by fluorescence in situ 
      hybridization (FISH). We also apply silver staining to the same individuals in 
      order to determine if structural changes are involved in the heteromorphism 
      detected by this technique. Our results show that rDNA loci map on the telomeric 
      region of the long arm of two submetacentric-subtelocentric chromosome pairs. In 
      addition to variations in NOR expression, we found some cases of structural 
      variations that affect the number of rDNA loci between individuals and the 
      location of the rDNA locus between the cells of the individual. We suggest that 
      FISH should be applied to other bivalves to assess the variation of rDNA loci and 
      undertake more accurate interspecific comparisons.
FAU - Insua, A
AU  - Insua A
AD  - Departamento de Biologia Celular y Molecular, Universidad de A Coruna, Spain.
FAU - Mendez, J
AU  - Mendez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 28S)
SB  - IM
MH  - Animals
MH  - Bivalvia/*genetics
MH  - *Chromosome Mapping
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 18S/*genetics
MH  - RNA, Ribosomal, 28S/*genetics
MH  - Silver Staining
EDAT- 1998/10/07 00:00
MHDA- 1998/10/07 00:01
CRDT- 1998/10/07 00:00
PHST- 1998/10/07 00:00 [pubmed]
PHST- 1998/10/07 00:01 [medline]
PHST- 1998/10/07 00:00 [entrez]
AID - 10.1111/j.1601-5223.1998.00189.x [doi]
PST - ppublish
SO  - Hereditas. 1998;128(3):189-94. doi: 10.1111/j.1601-5223.1998.00189.x.