PMID- 9761734
OWN - NLM
STAT- MEDLINE
DCOM- 19981222
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 335 ( Pt 2)
IP  - Pt 2
DP  - 1998 Oct 15
TI  - Phosphatidylinositol 4-kinase, but not phosphatidylinositol 3-kinase, is present 
      in GLUT4-containing vesicles isolated from rat skeletal muscle.
PG  - 351-6
AB  - Insulin stimulates the rate of glucose transport into muscle and adipose cells by 
      translocation of glucose transporter (GLUT4)-containing vesicles from an 
      intracellular storage pool to the surface membrane. This event is mediated 
      through the insulin receptor substrates (IRSs), which in turn activate 
      phosphatidylinositol (PI) 3-kinase isoforms. It has been suggested that insulin 
      causes attachment of PI 3-kinases to the intracellular GLUT4-containing vesicles 
      in rat adipose cells. Furthermore, it has also been shown that GLUT4-containing 
      vesicles in adipose cells contain a PI 4-kinase. In the present study we 
      investigate whether GLUT4-containing vesicles isolated from rat skeletal muscle 
      display PI 3-kinase and/or PI 4-kinase activities. Insulin stimulation caused a 
      rapid increase (5-15-fold increase compared with control) in the intracellular 
      cytosolic IRS-1-associated PI-3 kinase activity. This PI 3-kinase activity was 
      also present in a membrane preparation containing the insulin-regulatable pool of 
      GLUT4 transporters. However, when GLUT4-containing vesicles were isolated by 
      immunoprecipitation from basal and insulin-stimulated (3 min) skeletal muscle, 
      the vesicles displayed PI 4-kinase, but not PI 3-kinase, activity. Insulin did 
      not regulate the PI 4-kinase activity in the GLUT4-containing vesicles. In 
      conclusion, GLUT4-containing vesicles from rat skeletal muscle contain a PI 
      4-kinase, but not a PI 3-kinase. It is suggested that, in skeletal muscle, 
      insulin causes activation of the IRS/PI 3-kinase complex in an intracellular 
      membrane compartment associated closely with the GLUT4-containing vesicles, but 
      not in the GLUT4-containing vesicles themselves.
FAU - Kristiansen, S
AU  - Kristiansen S
AD  - Copenhagen Muscle Research Centre, August Krogh Institute, 13 Universitetsparken, 
      DK-2100 Copenhagen, Denmark. Skristiansen@aki.ku.dk
FAU - Ramlal, T
AU  - Ramlal T
FAU - Klip, A
AU  - Klip A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Androstadienes)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Insulin)
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, rat)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Slc2a4 protein, rat)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.67 (1-Phosphatidylinositol 4-Kinase)
RN  - K72T3FS567 (Adenosine)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
MH  - 1-Phosphatidylinositol 4-Kinase/drug effects/*metabolism
MH  - Adenosine/pharmacology
MH  - Androstadienes/pharmacology
MH  - Animals
MH  - Cell Membrane/chemistry/metabolism
MH  - Glucose Transporter Type 4
MH  - Insulin/pharmacology
MH  - Insulin Receptor Substrate Proteins
MH  - Male
MH  - Monosaccharide Transport Proteins/analysis/*metabolism
MH  - *Muscle Proteins
MH  - Muscle, Skeletal/drug effects/*metabolism
MH  - Phosphatidylinositol 3-Kinases/drug effects/*metabolism
MH  - Phosphoproteins/metabolism
MH  - Precipitin Tests
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Wortmannin
PMC - PMC1219789
EDAT- 1998/10/08 00:00
MHDA- 1998/10/08 00:01
PMCR- 1999/04/15
CRDT- 1998/10/08 00:00
PHST- 1998/10/08 00:00 [pubmed]
PHST- 1998/10/08 00:01 [medline]
PHST- 1998/10/08 00:00 [entrez]
PHST- 1999/04/15 00:00 [pmc-release]
AID - 10.1042/bj3350351 [doi]
PST - ppublish
SO  - Biochem J. 1998 Oct 15;335 ( Pt 2)(Pt 2):351-6. doi: 10.1042/bj3350351.