PMID- 9763572
OWN - NLM
STAT- MEDLINE
DCOM- 19981109
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 92
IP  - 8
DP  - 1998 Oct 15
TI  - DNA fiber fluorescence in situ hybridization analysis of immunoglobulin class 
      switching in B-cell neoplasia: aberrant CH gene rearrangements in follicle 
      center-cell lymphoma.
PG  - 2871-8
AB  - Immunoglobulin class switching usually involves deletion of part of the 
      immunoglobulin CH region. By DNA fiber fluorescence in situ hybridization (FISH) 
      with a barcode of probes covering the DH, JH, and CH genes, the configuration of 
      the entire CH region can be visualized on single DNA molecules. Using this 
      technique, we have studied class switching in three types of B-cell neoplasia, 
      mantle-cell lymphoma (MCL), follicular lymphoma (FL) and hairy cell leukemia 
      (HCL), representing B cells in, respectively, pregerminal center, germinal 
      center, and postgerminal center stages of development. In MCL and FL, 
      simultaneous detection of the t(11;14) and t(14;18) breakpoint with probes for 
      the BCL-1 and BCL-2 loci, respectively, allowed differentiation between 
      productive and nonproductive alleles. In none of 10 MCL cases was class switching 
      detected. In 21 HCL, all nonimmunoglobulin M (IgM) cases had class-switch 
      deletion consistent with the expressed isotype on at least one allele. In FL, 
      however, a peculiar pattern of CH rearrangement was observed. In IgM expressing 
      FL, the translocated alleles had switched in 11 of 13 cases, and the 
      nontranslocated allele showed complex rearrangements downstream from the 
      Cmu-Cdelta genes in 9 of 13 cases. These downstream rearrangements may reflect 
      tumor-specific deregulation of the class-switch machinery. All seven 
      immunoglobulin G (IgG) expressing FL showed class switching on both alleles. 
      Fiber FISH analysis also showed several polymorphisms. The most frequent one, 
      present on 38% of all analyzed alleles, consisted of an extra Cgamma gene or 
      pseudogene in the 3' cluster.
CI  - Copyright 1998 by The American Society of Hematology.
FAU - Vaandrager, J W
AU  - Vaandrager JW
AD  - Department of Pathology, Hematology, Cytometry and Cytochemistry, Leiden 
      University Medical Centre, Leiden, The Netherlands. 
      Jvaandrage@Path_1.MedFac.LeidenUniv.NL
FAU - Schuuring, E
AU  - Schuuring E
FAU - Kluin-Nelemans, H C
AU  - Kluin-Nelemans HC
FAU - Dyer, M J
AU  - Dyer MJ
FAU - Raap, A K
AU  - Raap AK
FAU - Kluin, P M
AU  - Kluin PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Immunoglobulin Constant Regions)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - B-Lymphocyte Subsets/*immunology/pathology
MH  - Biotinylation
MH  - Digoxigenin
MH  - *Gene Rearrangement, B-Lymphocyte, Heavy Chain
MH  - Genes, Immunoglobulin
MH  - Haplotypes/genetics
MH  - Humans
MH  - *Immunoglobulin Class Switching
MH  - Immunoglobulin Constant Regions/*genetics
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Follicular
EDAT- 1998/10/09 00:00
MHDA- 1998/10/09 00:01
CRDT- 1998/10/09 00:00
PHST- 1998/10/09 00:00 [pubmed]
PHST- 1998/10/09 00:01 [medline]
PHST- 1998/10/09 00:00 [entrez]
AID - S0006-4971(20)76207-5 [pii]
PST - ppublish
SO  - Blood. 1998 Oct 15;92(8):2871-8.