PMID- 9763574
OWN - NLM
STAT- MEDLINE
DCOM- 19981109
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 92
IP  - 8
DP  - 1998 Oct 15
TI  - Fluorescence in situ hybridization of progenitor cells obtained by 
      fluorescence-activated cell sorting for the detection of cells affected by 
      chromosome abnormality trisomy 8 in patients with myelodysplastic syndromes.
PG  - 2886-92
AB  - Myelodysplastic syndrome (MDS) is believed to be a stem-cell disorder involving 
      cytopenia and dysplastic changes in three hematopoietic lineages. However, the 
      involvement of pluripotent stem cells and progenitor cells has not been clarified 
      conclusively. To address this issue, we used fluorescence in situ hybridization 
      (FISH) of blood and bone marrow (BM) smears for mature cells and FISH of cells 
      sorted by fluorescence-activated cell sorting for progenitor cells. Seven 
      patients with MDS associated with trisomy 8 were studied. FISH showed +8 in 
      granulocytes, monocytes, and erythroblasts, but not in lymphocytes. Sorted cells 
      of T (CD3(+)), B (CD19(+)), and NK cells (CD3(-)CD56(+)) from peripheral blood 
      did not contain +8, nor did CD34(+) subpopulations from BM including B 
      (CD34(+)CD19(+)), T/NK (CD34(+)CD7(+)) progenitors, and pluripotent stem cells 
      (CD34(+)Thy1(+)). The +8 chromosome abnormality was identified in stem cells only 
      at the level of colony-forming unit of 
      granulocyte-erythrocyte-macrophage-megakaryocyte (CFU-GEMM; CD34(+)CD33(+)). It 
      may thus be concluded that cells affected by trisomy 8 in the context of MDS are 
      at the CFU-GEMM level and that cells of lymphoid lineage are not involved. These 
      results provide new insights into the biology of MDS and suggest that intensive 
      chemotherapy and autologous BM transplantation may become important therapeutic 
      strategies.
CI  - Copyright 1998 by The American Society of Hematology.
FAU - Saitoh, K
AU  - Saitoh K
AD  - Third Department of Internal Medicine, Akita University School of Medicine, 
      Akita, Japan.
FAU - Miura, I
AU  - Miura I
FAU - Takahashi, N
AU  - Takahashi N
FAU - Miura, A B
AU  - Miura AB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD19)
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antigens, CD19/analysis
MH  - Antigens, CD34/analysis
MH  - Bone Marrow/pathology
MH  - Cell Lineage
MH  - *Cell Separation
MH  - *Chromosomes, Human, Pair 8
MH  - Colony-Forming Units Assay
MH  - Female
MH  - *Flow Cytometry
MH  - Hematopoietic Stem Cells/pathology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*genetics
MH  - *Trisomy
EDAT- 1998/10/09 00:00
MHDA- 1998/10/09 00:01
CRDT- 1998/10/09 00:00
PHST- 1998/10/09 00:00 [pubmed]
PHST- 1998/10/09 00:01 [medline]
PHST- 1998/10/09 00:00 [entrez]
AID - S0006-4971(20)76209-9 [pii]
PST - ppublish
SO  - Blood. 1998 Oct 15;92(8):2886-92.