PMID- 9763661
OWN - NLM
STAT- MEDLINE
DCOM- 19981106
LR  - 20180213
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 82
IP  - 1-2
DP  - 1998
TI  - Position effect of translocations involving the inactive X chromosome: physical 
      linkage to XIC/XIST does not lead to long-range de novo inactivation in human 
      differentiated cells.
PG  - 58-66
AB  - Given the reported long-range cis-inactivating effect of the XIST gene in early 
      embryonic development and the lack of requirement of X-chromosome-specific 
      elements for propagating the inactive state, there exists the possibility of cis 
      inactivation of autosomal material after de novo translocation to an inactive X 
      chromosome (Xi) in differentiated cells. We have analyzed de novo 
      radiation-induced translocations between the Xi and autosomes to study the 
      maintenance and spreading of X-chromosome inactivation (X inactivation) in 
      relation to the position of the X-inactivation center (XIC)/XIST in 
      differentiated cells. Autosome/Xi translocations were detected by fluorescence in 
      situ hybridization (FISH). The activation status of the chromosomes involved in 
      the translocation was determined by simultaneous immunocytogenetic studies using 
      antibodies against either BrdU incorporated at late S phase or acetylated histone 
      H4. The position of XIC/XIST in the reciprocal products of the translocation was 
      determined by XIST-specific FISH and computer enhancement. In other experiments, 
      the Xq13 region carrying XIC/XIST was localized by computer enhancement of the 
      DAPI banding pattern. Our study in differentiated cells provides a visual 
      demonstration that physical separation from XIC/XIST does not result in 
      reactivation of inactive X-chromosome material and that X inactivation is not 
      spread to the translocated autosomes irrespective of the position of XIC/XIST. 
      This observation suggests that physical linkage to XIC/XIST does not lead to de 
      novo inactivation of autosomal material.
FAU - Surralles, J
AU  - Surralles J
AD  - Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      Leiden, The Netherlands. jordi.surralles@blues.uab.es
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
RN  - 0 (Transcription Factors)
RN  - 0 (XIST non-coding RNA)
SB  - IM
MH  - Cell Differentiation/genetics
MH  - Cell Line
MH  - *Dosage Compensation, Genetic
MH  - *Genetic Linkage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - RNA, Long Noncoding
MH  - *RNA, Untranslated
MH  - Transcription Factors/*genetics
MH  - *Translocation, Genetic
EDAT- 1998/10/09 02:00
MHDA- 2000/08/16 11:00
CRDT- 1998/10/09 02:00
PHST- 1998/10/09 02:00 [pubmed]
PHST- 2000/08/16 11:00 [medline]
PHST- 1998/10/09 02:00 [entrez]
AID - ccg82058 [pii]
AID - 10.1159/000015065 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1998;82(1-2):58-66. doi: 10.1159/000015065.