PMID- 9764362
OWN - NLM
STAT- MEDLINE
DCOM- 19981202
LR  - 20231213
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 40
IP  - 3
DP  - 1998 Sep
TI  - Unique biochemical properties of human leukocyte antigen-E allow for a highly 
      specific function in immune recognition.
PG  - 177-82
AB  - PROBLEM: Does a correlation exist between the expression of human leukocyte 
      antigen (HLA) class Ia and HLA-E and what is its biological significance? METHOD 
      OF STUDY: HLA-E transcripts were detected by reverse transcriptase-polymerase 
      chain reaction. Metabolically labeled HLA-E heavy chains were immunoprecipited 
      and analyzed by one-dimensional isoelectric focusing. Mouse RMA-S cells defective 
      with regard to transporter associated with antigen processing (TAP) function were 
      transfected with HLA-E and human beta 2-microglobulin to investigate TAP 
      dependence of the cell-surface expression of HLA-E. RESULTS: HLA-E is transcribed 
      regardless of the down-regulation of polymorphic HLA class Ia expression. HLA-E 
      is transported to the cell surface in the absence of TAP-controlled peptide 
      loading. In human cells, the amount of HLA-E protein is very low regardless of 
      the presence of correct peptide ligands. CONCLUSIONS: HLA-E regulates immune 
      functions in cells that have down-regulated the expression of polymorphic 
      HLA-class Ia molecules, either by preventing harmful natural killer cells from 
      attacking targets that have physiologically decreased HLA-class Ia expression or 
      by activating effector cells against virus-infected and tumor cells with impaired 
      HLA-class Ia expression.
FAU - Weiss, E H
AU  - Weiss EH
AD  - Institut fur Anthropologie und Humangenetik, Ludwig-Maximilians-Universitat, 
      Munchen, Germany.
FAU - Cannich, A
AU  - Cannich A
FAU - Sprinks, M
AU  - Sprinks M
FAU - Fernandez, N
AU  - Fernandez N
FAU - Ulbrecht, M
AU  - Ulbrecht M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - HLA Antigens/biosynthesis/genetics/*immunology
MH  - Histocompatibility Antigens Class I/biosynthesis/genetics/*immunology
MH  - Humans
MH  - Isoelectric Focusing
MH  - Mice
MH  - Precipitin Tests
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Transfection
MH  - HLA-E Antigens
EDAT- 1998/10/09 00:00
MHDA- 1998/10/09 00:01
CRDT- 1998/10/09 00:00
PHST- 1998/10/09 00:00 [pubmed]
PHST- 1998/10/09 00:01 [medline]
PHST- 1998/10/09 00:00 [entrez]
AID - 10.1111/j.1600-0897.1998.tb00410.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 1998 Sep;40(3):177-82. doi: 
      10.1111/j.1600-0897.1998.tb00410.x.