PMID- 9764601
OWN - NLM
STAT- MEDLINE
DCOM- 19981023
LR  - 20240322
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 114
IP  - 1
DP  - 1998 Oct
TI  - Evidence of idiotypic modulation in the immune response to gp43, the major 
      antigenic component of Paracoccidioides brasiliensis in both mice and humans.
PG  - 40-8
AB  - Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with 
      a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic 
      agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A 
      glycoprotein of 43,000 D (gp43) is the major antigen of P. brasiliensis. 
      Antibodies directed to this antigen are detected in the sera of all patients with 
      PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and 
      pathogenesis of the fungus. As the role of antibodies in PCM is not fully 
      understood, we decided to investigate the outcome of mice immunization with three 
      distinct anti-gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet 
      haemocyanin (KLH). Results show not only the expected presence of anti-Id (AB2) 
      antibodies in the sera of these animals but also a spontaneous and increasing 
      amount of anti-anti-Id (AB3) antibodies after the third course of immunization. 
      Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from 
      mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of 
      mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' 
      sera with high titres of anti-gp43 antibodies generate anti-Id antibodies. These 
      data suggest that the immune response to P. brasiliensis can be spontaneously 
      modulated by the idiotypic network.
FAU - Souza, A R
AU  - Souza AR
AD  - Microbiology, Immunology and Parasitology Department, Federal University of Sao 
      Paulo (UNIFESP), Brazil.
FAU - Gesztesi, J L
AU  - Gesztesi JL
FAU - Moraes, J Z
AU  - Moraes JZ
FAU - Cruz, C R
AU  - Cruz CR
FAU - Sato, J
AU  - Sato J
FAU - Mariano, M
AU  - Mariano M
FAU - Lopes, J D
AU  - Lopes JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 0 (Oligosaccharides)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Antibodies, Fungal/immunology
MH  - Antibodies, Monoclonal/immunology
MH  - Antigens, Fungal/immunology
MH  - Carcinoembryonic Antigen/immunology
MH  - Female
MH  - *Fungal Proteins
MH  - Glycoproteins/*immunology
MH  - Humans
MH  - Hybridomas
MH  - Immunization, Passive
MH  - Immunoglobulin Idiotypes/blood/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligosaccharides/*immunology
MH  - Paracoccidioides/immunology
MH  - Paracoccidioidomycosis/blood/*immunology
PMC - PMC1905082
EDAT- 1998/10/09 00:00
MHDA- 1998/10/09 00:01
PMCR- 1999/10/01
CRDT- 1998/10/09 00:00
PHST- 1998/10/09 00:00 [pubmed]
PHST- 1998/10/09 00:01 [medline]
PHST- 1998/10/09 00:00 [entrez]
PHST- 1999/10/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2249.1998.00679.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 1998 Oct;114(1):40-8. doi: 10.1046/j.1365-2249.1998.00679.x.