PMID- 9766751
OWN - NLM
STAT- MEDLINE
DCOM- 19981020
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 22
IP  - 10
DP  - 1998 Oct
TI  - Introduction of a normal retinoblastoma (Rb) gene into Rb-deficient 
      lymphoblastoid cells delays tumorigenicity in immunodefective mice.
PG  - 905-12
AB  - Inactivation of the Rb susceptibility gene occurs in various human cancers and 
      has been associated with tumorigenicity. Rb gene is inactivated in 30% of acute 
      leukaemias. The effect of Rb protein expression was assessed in the 
      lymphoblastoid cell line IM-9 defective for Rb protein, after stable transfection 
      with a wild-type Rb gene. The Rb transgene was under the control of the 
      MoMuLv-LTR. Protein expression by the transduced cells was confirmed by Western 
      blot and flow cytometry analyses. Compared to the parental cell line, growth rate 
      remained unchanged in the Rb transfected clones. In SCID mice however, tumor 
      formation originating from these clones was delayed. The current data suggest 
      therefore that, in this Rb-defective haematopoietic cell line, Rb expression 
      correlates with reduced tumorigenicity but not with reduced growth rate.
FAU - Lefebvre, D
AU  - Lefebvre D
AD  - Laboratoire de Biologie Moleculaire, Cliniques Universitaires Saint Luc, 
      University of Louvain, Brussels, Belgium.
FAU - Gala, J L
AU  - Gala JL
FAU - Heusterspreute, M
AU  - Heusterspreute M
FAU - Delhez, H
AU  - Delhez H
FAU - Philippe, M
AU  - Philippe M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (DNA, Neoplasm)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Animals
MH  - *B-Lymphocytes
MH  - DNA, Neoplasm/genetics
MH  - *Genes, Retinoblastoma
MH  - *Genetic Therapy
MH  - Humans
MH  - Mice
MH  - Mice, SCID
MH  - Multiple Myeloma/immunology
MH  - Phenotype
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Neoplasm/genetics
MH  - Severe Combined Immunodeficiency/genetics/*therapy
MH  - Time Factors
MH  - Transfection
MH  - Tumor Cells, Cultured
EDAT- 1998/10/10 00:00
MHDA- 1998/10/10 00:01
CRDT- 1998/10/10 00:00
PHST- 1998/10/10 00:00 [pubmed]
PHST- 1998/10/10 00:01 [medline]
PHST- 1998/10/10 00:00 [entrez]
AID - S0145212698000794 [pii]
AID - 10.1016/s0145-2126(98)00079-4 [doi]
PST - ppublish
SO  - Leuk Res. 1998 Oct;22(10):905-12. doi: 10.1016/s0145-2126(98)00079-4.