PMID- 9767412
OWN - NLM
STAT- MEDLINE
DCOM- 19981019
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 94
IP  - 3
DP  - 1998 Jul
TI  - Specific antigen targeting to surface IgE and IgG on mouse bone marrow-derived 
      mast cells enhances efficiency of antigen presentation.
PG  - 318-24
AB  - The discovery that bone marrow-derived mast cells can express major 
      histocompatibility complex class II molecules and act as antigen-presenting cells 
      prompted us to evaluate this function when antigen is internalized through 
      fluid-phase endocytosis or via specific uptake by using IgG and IgE antibodies. 
      This study was performed using a specific T-cell hybridoma developed against Lol 
      p 1, the major allergen of grass pollen Lolium perenne. Expression of Fc gamma R 
      and Fc epsilon RI by mast cells led us to investigate the influence of IgG- and 
      IgE-targeted antigen on the antigen-presenting function of mast cells. 
      Internalization of Lol p 1 through different specific IgG monoclonal antibodies 
      (mAb) resulted in the activation of Lol p 1-specific T-cell hybridoma at 
      concentrations about 100-fold less than that required for T-cell stimulation by 
      uncomplexed antigen. IgE-complexed Lol p 1, which facilitates trapping of antigen 
      by mast cells, induced an accelerated and more efficient antigen-presenting 
      capacity of mast cells than that obtained with uncomplexed antigen. However, 
      aggregation of anti-dinitrophenyl (DNP) IgE mAb by the irrelevant antigen 
      DNP-human serum albumin did not substantially increase the capacity of mast cells 
      to present Lol p 1 to T cells. This suggests that the mere aggregation of Fc 
      epsilon RI is not sufficient for enhanced antigen presentation mediated by IgE. 
      Tissue distribution and strategic location of mast cells at the mucosal barriers 
      and their capacity to process the antigen through efficient fluid-phase 
      pinocytosis as well as IgG- and IgE-dependent targeting of antigens provide mast 
      cells with a prominent role in immune surveillance.
FAU - Tkaczyk, C
AU  - Tkaczyk C
AD  - Unite d'Immunoallergie, Institut Pasteur, Paris, France.
FAU - Viguier, M
AU  - Viguier M
FAU - Boutin, Y
AU  - Boutin Y
FAU - Frandji, P
AU  - Frandji P
FAU - David, B
AU  - David B
FAU - Hebert, J
AU  - Hebert J
FAU - Mecheri, S
AU  - Mecheri S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Allergens)
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Plant)
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Immunotoxins)
RN  - 0 (Lol p I protein, Lolium perenne)
RN  - 0 (Plant Proteins)
RN  - 0 (Receptors, IgE)
RN  - 0 (Receptors, IgG)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens
MH  - Animals
MH  - Antibodies/immunology
MH  - Antigen Presentation/*physiology
MH  - Antigens, Plant
MH  - Bone Marrow Cells/*immunology
MH  - Dinitrobenzenes/immunology
MH  - Hybridomas
MH  - Immunoglobulin E/immunology
MH  - Immunotoxins/pharmacology
MH  - Lolium
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Pinocytosis
MH  - Plant Proteins
MH  - Receptors, IgE/*immunology
MH  - Receptors, IgG/*immunology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
PMC - PMC1364248
EDAT- 1998/10/10 00:00
MHDA- 1998/10/10 00:01
PMCR- 1999/07/01
CRDT- 1998/10/10 00:00
PHST- 1998/10/10 00:00 [pubmed]
PHST- 1998/10/10 00:01 [medline]
PHST- 1998/10/10 00:00 [entrez]
PHST- 1999/07/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1998.00525.x [doi]
PST - ppublish
SO  - Immunology. 1998 Jul;94(3):318-24. doi: 10.1046/j.1365-2567.1998.00525.x.