PMID- 9768618 OWN - NLM STAT- MEDLINE DCOM- 19981015 LR - 20191024 IS - 1077-9450 (Print) IS - 1077-9450 (Linking) VI - 19 IP - 2 DP - 1998 Oct 1 TI - Effective lysis of HIV-1-infected primary CD4+ T cells by a cytotoxic T-lymphocyte clone directed against a novel A2-restricted reverse-transcriptase epitope. PG - 111-20 AB - Most HIV-specific cytotoxic T-lymphocyte (CTL) epitopes have been identified using peptide-pulsed and recombinant vaccinia virus-infected targets. These systems may not accurately reflect the ability of epitopes to be presented by HIV-infected T cells. Recent studies suggest, in fact, that some CTL epitopes are poorly presented on HIV-infected cells. In this study, we have identified a novel A2.1-restricted HIV reverse-transcriptase (RT) epitope and investigated the presentation of this epitope by HIV-infected primary CD4+ T cells and T-cell lines. A CD8+ CTL clone, isolated from a seropositive subject that recognized a novel A2-restricted epitope KYTAFTIPSI (aa 293-302) in RT, was used for these studies. Primary CD4+ T cells and the CD4+ T-cell line T1 were infected with virus from T1-nPLAP, a cell line stably transfected with HXB-nPLAP, a molecular construct of HIV linked to a placental alkaline phosphatase (PLAP) marker gene. A uniformly infected cell population, obtained by immunomagnetic selection for PLAP expression, was used as targets in CTL assays. HIV-infected T cells were lysed by CTL recognizing this RT epitope as effectively as peptide-pulsed targets. This suggests that some RT epitopes are good targets for CTL recognition. FAU - Shankar, P AU - Shankar P AD - The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA. FAU - Sprang, H AU - Sprang H FAU - Lieberman, J AU - Lieberman J LA - eng GR - R29 AI38819/AI/NIAID NIH HHS/United States PT - Journal Article PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Acquir Immune Defic Syndr Hum Retrovirol JT - Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association JID - 9501482 RN - 0 (Epitopes) RN - EC 2.7.7.49 (HIV Reverse Transcriptase) SB - IM MH - Amino Acid Sequence MH - CD4-Positive T-Lymphocytes/*immunology/virology MH - Cell Line, Transformed MH - Cytotoxicity Tests, Immunologic MH - Cytotoxicity, Immunologic MH - Epitope Mapping MH - Epitopes/chemistry/*immunology MH - Flow Cytometry MH - HIV Reverse Transcriptase/chemistry/*immunology MH - HIV Seropositivity/*immunology/pathology MH - HIV-1/enzymology/*immunology MH - Humans MH - Microspheres MH - T-Lymphocytes, Cytotoxic/*immunology/virology MH - Transfection MH - Tumor Cells, Cultured MH - Up-Regulation EDAT- 1998/10/13 00:00 MHDA- 1998/10/13 00:01 CRDT- 1998/10/13 00:00 PHST- 1998/10/13 00:00 [pubmed] PHST- 1998/10/13 00:01 [medline] PHST- 1998/10/13 00:00 [entrez] AID - 10.1097/00042560-199810010-00002 [doi] PST - ppublish SO - J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Oct 1;19(2):111-20. doi: 10.1097/00042560-199810010-00002.