PMID- 9769892
OWN - NLM
STAT- MEDLINE
DCOM- 19981116
LR  - 20041117
IS  - 0369-8114 (Print)
IS  - 0369-8114 (Linking)
VI  - 46
IP  - 5
DP  - 1998 May
TI  - Pseudomonas aeruginosa from cystic fibrosis patients: study using whole cell RAPD 
      and antibiotic susceptibility.
PG  - 319-24
AB  - Pseudomonas aeruginosa is the most important bacterial pathogen in lung disease 
      of cystic fibrosis patients. Different morphotypes of the bacterium are 
      frequently recovered in sputum samples of these patients. We developed a whole 
      cell Randomly Amplified Polymorphic DNA (RAPD) technique in order to establish 
      the relatedness between morphotype, genotype and antibiotic susceptibility. Six 
      cystic fibrosis patients already colonized by P. aeruginosa were investigated by 
      collecting three successive sputum samples (before and after antibiotic 
      treatment, and one month later) and selecting 10 isolates per morphotype. 250 
      isolates of P. aeruginosa were recovered from 16 of 18 sputum samples. Five 
      patients carried a single RAPD type strain four of which showed at least two 
      morphotypes; one patient carried two RAPD types strains. No patients carried the 
      same strain. These results confirmed other studies previously published in 
      showing stability of the chronic colonization with a single strain. Antibiotype 
      differences were not associated with differences of RAPD profiles and no relation 
      was found between antibiotype and morphotype.
FAU - Mereghetti, L
AU  - Mereghetti L
AD  - Laboratoire de Bacteriologie et d'Hygiene Hospitaliere, Hopital Trousseau, CHU de 
      Tours, France.
FAU - Marquet-van der Mee, N
AU  - Marquet-van der Mee N
FAU - Loulergue, J
AU  - Loulergue J
FAU - Rolland, J C
AU  - Rolland JC
FAU - Audurier, A
AU  - Audurier A
LA  - eng
PT  - Journal Article
PL  - France
TA  - Pathol Biol (Paris)
JT  - Pathologie-biologie
JID - 0265365
SB  - IM
MH  - Cystic Fibrosis/*complications
MH  - *Drug Resistance, Microbial
MH  - Female
MH  - Humans
MH  - Male
MH  - Opportunistic Infections/drug therapy/*microbiology
MH  - Pneumonia, Bacterial/drug therapy/*microbiology
MH  - Pseudomonas Infections/drug therapy/*microbiology
MH  - Pseudomonas aeruginosa/drug effects/genetics/*isolation & 
      purification/ultrastructure
MH  - *Random Amplified Polymorphic DNA Technique
MH  - Sputum/microbiology
EDAT- 1998/10/14 00:00
MHDA- 1998/10/14 00:01
CRDT- 1998/10/14 00:00
PHST- 1998/10/14 00:00 [pubmed]
PHST- 1998/10/14 00:01 [medline]
PHST- 1998/10/14 00:00 [entrez]
PST - ppublish
SO  - Pathol Biol (Paris). 1998 May;46(5):319-24.