PMID- 9770435
OWN - NLM
STAT- MEDLINE
DCOM- 19981105
LR  - 20061115
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 250
IP  - 1
DP  - 1998 Oct 10
TI  - The 41-kDa protein of human herpesvirus 6 specifically binds to viral DNA 
      polymerase and greatly increases DNA synthesis.
PG  - 210-9
AB  - We previously isolated a 41-kDa early antigen of human herpesvirus 6 (HHV-6), 
      which exhibited nuclear localization and DNA-binding activity (Agulnick et al., 
      1993). In this study, we observed that a 110-kDa protein was coimmunoprecipitated 
      with p41 from HHV-6-infected cells by an anti-p41 antibody. This 110-kDa protein 
      was identified as the HHV-6 DNA polymerase (Pol-6) by an antibody raised against 
      the N terminus of Pol-6. Reciprocal immunoprecipitation and Western blot analyses 
      confirmed that p41 complexes with Pol-6 in HHV-6-infected cells. In addition, 
      both p41 and Pol-6 were expressed in vitro and shown to form a specific complex. 
      An in vitro DNA synthesis assay using primed M13 single-stranded DNA template 
      demonstrated that p41 not only increased the DNA synthesis activity of Pol-6 but 
      also allowed Pol-6 to synthesize DNA products corresponding to full-length M13 
      template (7249 nucleotides). By contrast, Pol-6 alone could only synthesize DNA 
      of <100 nucleotides. The functional interaction between Pol-6 and p41 appears to 
      be specific because they could not be physically or functionally substituted in 
      vitro by their herpes simplex virus 1 homologues. Moreover, as revealed by 
      mutational analysis, both the N and C termini of Pol-6 contribute to its binding 
      to p41. In the case of p41, the N terminus is required for increasing DNA 
      synthesis but not binding to Pol-6, whereas the C terminus is totally 
      dispensable.
CI  - Copyright 1998 Academic Press.
FAU - Lin, K
AU  - Lin K
AD  - School of Dental Medicine, School of Medicine, University of Pennsylvania, 4010 
      Locust Street, Philadelphia, Pennsylvania, 19104, USA.
FAU - Ricciardi, R P
AU  - Ricciardi RP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Viral Proteins)
RN  - 138415-18-6 (p41 protein, Human herpesvirus 6)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Cell Line
MH  - DNA, Viral/*biosynthesis
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - DNA-Directed DNA Polymerase/genetics/*metabolism
MH  - Herpesvirus 6, Human/enzymology/*metabolism
MH  - Humans
MH  - Protein Binding
MH  - Sequence Deletion
MH  - T-Lymphocytes/virology
MH  - Viral Proteins/genetics/*metabolism
EDAT- 1998/10/15 00:00
MHDA- 1998/10/15 00:01
CRDT- 1998/10/15 00:00
PHST- 1998/10/15 00:00 [pubmed]
PHST- 1998/10/15 00:01 [medline]
PHST- 1998/10/15 00:00 [entrez]
AID - S0042-6822(98)99348-5 [pii]
AID - 10.1006/viro.1998.9348 [doi]
PST - ppublish
SO  - Virology. 1998 Oct 10;250(1):210-9. doi: 10.1006/viro.1998.9348.