PMID- 977219
OWN - NLM
STAT- MEDLINE
DCOM- 19761223
LR  - 20131121
IS  - 0300-9831 (Print)
IS  - 0300-9831 (Linking)
VI  - 46
IP  - 3
DP  - 1976
TI  - Retinyl palmitate, a major retinyl ester in rat testis seminiferous tubules. II. 
      The Sertoli cell, site of retinol esterification.
PG  - 330-7
AB  - The study was designed to 1) isolate and quantitate retinyl esters normally 
      present in the testis seminiferous tubules (ST) and 2) determine whether the 
      Sertoli (SE) cells in the ST have the enzyme system necessary to esterify 
      retinol. In the first study testes from normal adult male rats were removed, the 
      ST were isolated mechanically and retinol was extracted and separated into free 
      and retinyl esters in a prestandardized alumina column. The fraction containing 
      retinyl esters was hydrolyzed, and the fatty acid composition was determined gas 
      chromatographically. The results showed that retinyl palmitate was the major 
      retinyl ester in the testis ST constituting almost 55% of the total esters. In 
      the second study adult male rats were given Busulfan (1,4, 
      dimethylsulfonoxybutane) intraperitoneally twice six weeks apart. At the end of 
      six weeks following injection, the testes from a few animals were examined 
      histologically, and the results showed that Busulfan treatment destroyed the 
      germinal epithelium completely but had no effect on the structural integrity of 
      the SE cells. Labeled retinol (free) mixed in rat albumin solution was injected 
      intratesticularly (in vivo study) or incubated (in vitro study) with ST which had 
      been isolated from the testes of rats treated with Busulfan. The results of the 
      studies indicate that labeled retinol (free) was converted to retinyl esters in 
      the ST. Since the ratio of conversion of free retinol (labeled) to retinyl esters 
      remained constant in the treated ST (which contained only Sertoli cells, the 
      germinal epithelium having been destroyed) and in the control ST (which contained 
      both Sertoli cells and germinal epithelium), we conclude that the Sertoli cells 
      are the site of retinol esterification in the testis ST.
FAU - Ahluwalia, B
AU  - Ahluwalia B
FAU - Gambhir, K K
AU  - Gambhir KK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Int J Vitam Nutr Res
JT  - International journal for vitamin and nutrition research. Internationale 
      Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de 
      vitaminologie et de nutrition
JID - 1273304
RN  - 11103-57-4 (Vitamin A)
RN  - G1LN9045DK (Busulfan)
SB  - IM
MH  - Animals
MH  - Busulfan/pharmacology
MH  - Esterification
MH  - Male
MH  - Organ Size/drug effects
MH  - Rats
MH  - Seminiferous Tubules/*metabolism
MH  - Sertoli Cells/drug effects/*metabolism
MH  - Testis/anatomy & histology/drug effects/*metabolism
MH  - Vitamin A/*analogs & derivatives/analysis/metabolism
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
PST - ppublish
SO  - Int J Vitam Nutr Res. 1976;46(3):330-7.