PMID- 9772283
OWN - NLM
STAT- MEDLINE
DCOM- 19981209
LR  - 20190516
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 13
IP  - 5
DP  - 1998 Nov
TI  - Effects of all-trans retinoic acid and interferon alpha in peripheral 
      neuroectodermal tumor cell cultures and xenografts.
PG  - 943-9
AB  - Peripheral neuroectodermal tumors (PNET) have an unsatisfactory outcome when 
      treated with standard approaches. Among novel treatments, the use of biological 
      response modifiers has rarely been reported in this group of malignancies. We 
      have previously demonstrated that both all-trans retinoic acid (ATRA) and 
      interferon a (IFNa) can inhibit proliferation of human PNET cells and that ATRA 
      can up-regulate IFNa receptor expression in vitro. In this study we evaluated the 
      anti-tumor effects of ATRA and IFNa in PNET cells in vitro and in a human PNET 
      xenograft model, using CHP100 cells. A synergistic inhibitory effect of ATRA and 
      IFNa was observed on CHP100 cells in vitro. On the contrary, a significant 
      inhibition of tumor growth was observed in mice treated with ATRA alone, whereas 
      neither IFNa nor the combination of ATRA and IFNa, reached a statistically 
      significant anti-tumor effect. Histologic examination of tumors revealed the 
      presence of necrosis upon treatment with IFNa, whereas almost no necrosis, but a 
      more differentiated morphology, confirmed by electron microscopy analysis, was 
      associated with the ATRA containing treatments. Taken together these data show an 
      in vitro and in vivo anti-tumor activity of ATRA in human PNET cells, although no 
      synergism of ATRA and IFNa was observed in our xenograft model.
FAU - Rosolen, A
AU  - Rosolen A
AD  - Divisione di Emato-Oncologia Pediatrica, Dipartimento di Pediatria, Universita di 
      Padova, 35128 Padova, Italy.
FAU - Favaretto, G
AU  - Favaretto G
FAU - Masarotto, G
AU  - Masarotto G
FAU - Cavazzana, A
AU  - Cavazzana A
FAU - Zanesco, L
AU  - Zanesco L
FAU - Frascella, E
AU  - Frascella E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Interferon-alpha)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Combined Chemotherapy Protocols/*pharmacology/therapeutic use
MH  - Cell Division/drug effects
MH  - Drug Synergism
MH  - Humans
MH  - Interferon-alpha/*pharmacology/therapeutic use
MH  - Mice
MH  - Mice, Nude
MH  - Neuroectodermal Tumors, Primitive, Peripheral/*drug 
      therapy/pathology/ultrastructure
MH  - Transplantation, Heterologous
MH  - Tretinoin/*pharmacology/therapeutic use
MH  - Tumor Cells, Cultured
EDAT- 1998/10/17 00:00
MHDA- 1998/10/17 00:01
CRDT- 1998/10/17 00:00
PHST- 1998/10/17 00:00 [pubmed]
PHST- 1998/10/17 00:01 [medline]
PHST- 1998/10/17 00:00 [entrez]
AID - 10.3892/ijo.13.5.943 [doi]
PST - ppublish
SO  - Int J Oncol. 1998 Nov;13(5):943-9. doi: 10.3892/ijo.13.5.943.