PMID- 9772905
OWN - NLM
STAT- MEDLINE
DCOM- 19981023
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 106
IP  - 1
DP  - 1998 Oct 1
TI  - Characterization of multiple 12p rearrangements in testicular germ cell tumor 
      cell line 833K and its subclone 64CP by chromosome microdissection.
PG  - 24-9
AB  - Rearrangements of chromosome arm 12p are known to be common in germ cell tumors 
      (GCT). Previous studies, using fluorescence in situ hybridization (FISH) with a 
      whole chromosome 12 painting probe, showed unusual distributions of chromosome 
      12-derived chromatin in GCT cell line 833K and its cisplatin-resistant subclone, 
      64CP, located next to AgNOR (silver staining nucleolus organizer regions), some 
      of which were ectopic. In this study, the ectopic stalk regions were shown by 
      FISH to be composed of 18s and 28s rDNA, but were flanked by beta-satellite DNA, 
      which may form a barrier around the rDNA. In order to determine the specific 
      origins of the rearranged chromosome 12 segments, three different derived 
      chromosome 12 regions were isolated from 64CP, using chromosomal microdissection. 
      The microdissected fragments were labeled and hybridized by FISH to normal human 
      chromosomes. All three segments localized to distal 12p; 12p12-->12pter, but with 
      apparently different breakpoints for each segment. Furthermore, three-color FISH 
      experiments with 12p band-specific probes demonstrated that the derivative 
      chromosome 12 regions in 833K also originate from distal 12p (12p12-->p13). These 
      sequences now can be evaluated for degree of overlap or common breakpoints which 
      may be of significance in the development or progression of GCT.
FAU - Blough, R I
AU  - Blough RI
AD  - Department of Medical and Molecular Genetics, Indiana University School of 
      Medicine, Indianapolis, USA.
FAU - Vance, G H
AU  - Vance GH
FAU - Henegariu, O
AU  - Henegariu O
FAU - Smolarek, T A
AU  - Smolarek TA
FAU - Sledge, G W Jr
AU  - Sledge GW Jr
FAU - Heerema, N A
AU  - Heerema NA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - Germinoma/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Testicular Neoplasms/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1998/10/17 02:11
MHDA- 2001/03/28 10:01
CRDT- 1998/10/17 02:11
PHST- 1998/10/17 02:11 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1998/10/17 02:11 [entrez]
AID - S0165460898000429 [pii]
AID - 10.1016/s0165-4608(98)00042-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Oct 1;106(1):24-9. doi: 
      10.1016/s0165-4608(98)00042-9.