PMID- 9773294
OWN - NLM
STAT- MEDLINE
DCOM- 19981217
LR  - 20231213
IS  - 0239-8508 (Print)
IS  - 0239-8508 (Linking)
VI  - 36
IP  - 3
DP  - 1998
TI  - Antiserum against connexin32 fragment reacts with a 32-kD protein localised in 
      gap junctions of mouse and rat liver, endometrium and in the fish heart.
PG  - 111-8
AB  - The expression of various connexins so far identified is metabolically and 
      developmentally regulated. Examples include uterine endometrium where the 
      expression of gap junction protein, connexin32 (Cx32) is regulated by steroid 
      hormones. In this study we attempted to synthesise a short peptide which matches 
      the portion of the amino acid sequence of the Cx32. Cx32 has primary structure 
      predicted from the nucleotide sequence of cDNA clone. A fragment of Cx32 molecule 
      was synthesised to produce anti-peptide antibody for detecting gap junction 
      protein in mouse and rat liver and endometrium. The 12-peptide, plus Abu residue 
      that corresponds to residues 108-119 (LEGHGDPLHLEE-Abu) of the rat Cx32 (283-mer) 
      was synthesised by the solid phase method. Antibodies against this peptide were 
      raised in rabbits, screened for reactivity and specificity using dot blot assays 
      [15] and used for immunocytochemical staining at the light and at electron 
      microscope levels. The antibodies also reacted with fish heart myocardium.
FAU - Kilarski, W M
AU  - Kilarski WM
AD  - Department of Cytology and Histology, Jagiellonian University, Krakow, Poland.
FAU - Witczuk, B
AU  - Witczuk B
FAU - Kupryszewski, G
AU  - Kupryszewski G
FAU - Semik, D
AU  - Semik D
FAU - Gorlich, A
AU  - Gorlich A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0 (Antibodies)
RN  - 0 (Connexins)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/metabolism
MH  - Connexins/*chemistry
MH  - Endometrium/*chemistry
MH  - Female
MH  - Fishes
MH  - Gap Junctions/*chemistry
MH  - Liver/*chemistry
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Myocardium/*chemistry
MH  - Peptide Fragments/*chemistry/immunology
MH  - Pregnancy
MH  - Rabbits
MH  - Rats
MH  - Gap Junction beta-1 Protein
EDAT- 1998/10/17 00:00
MHDA- 1998/10/17 00:01
CRDT- 1998/10/17 00:00
PHST- 1998/10/17 00:00 [pubmed]
PHST- 1998/10/17 00:01 [medline]
PHST- 1998/10/17 00:00 [entrez]
PST - ppublish
SO  - Folia Histochem Cytobiol. 1998;36(3):111-8.