PMID- 9778124
OWN - NLM
STAT- MEDLINE
DCOM- 19981209
LR  - 20190921
IS  - 0143-4004 (Print)
IS  - 0143-4004 (Linking)
VI  - 19
IP  - 7
DP  - 1998 Sep
TI  - Heme oxygenase and nitric oxide synthase in the placenta of the guinea-pig during 
      gestation.
PG  - 509-16
AB  - Nitric oxide (NO) and carbon monoxide (CO) are novel gaseous chemical messengers 
      that play key roles in cell function and cell-cell communication in many organ 
      systems, including the cardiovascular system. Although the presence of NO 
      synthase (NOS) in the placenta and its role in the regulation of fetoplacental 
      and uteroplacental blood flow are well established, little is known about 
      placental expression and activity of heme oxygenase (HO), the enzyme that 
      catalyses the oxidation of heme to CO, biliverdin and iron, during gestation. The 
      objectives of this study were to elucidate the localization of HO-1 and HO-2 
      isoforms relative to NOS III protein, and to determine the enzymatic activity of 
      HO in the placenta of the guinea-pig during gestation. Placentae were obtained 
      from pregnant guinea-pigs at gestational day (GD) 34, 50, 62 and full term (term, 
      about GD 68). Immunohistochemical localization of HO-1, HO-2 and NOS III protein 
      was conducted using selective polyclonal antibodies. HO activity was determined 
      by using a gas chromatographic method to measure the rate of formation of CO from 
      heme. Faint staining for HO-1 was observed in the adventitial layer of larger 
      fetal blood vessels of the placenta at GD 34. The intensity of this staining was 
      higher at GD 50 and GD 62, and decreased at full term. Similar areas in serial 
      sections of placentae obtained at these selected times during gestation exhibited 
      lower staining intensity when incubated with anti-HO-2 antiserum. Placental HO 
      activity was significantly increased (P<0.05) at GD 62 compared with GD 34, GD 50 
      and full term. NOS III (endothelial constitutive NOS) staining was highest at GD 
      34, decreasing thereafter, and was localized mostly to trophoblast lining 
      maternal channels. The data demonstrate that, in the guinea-pig, placental HO and 
      NOS differ in tissue localization during the second half of gestation, with 
      expression of HO protein and its catalytic activity being higher during near-term 
      pregnancy. In a preliminary immunohistochemical investigation of the full-term 
      human placenta, HO-1 protein was localized primarily in the adventitial region of 
      fetal blood vessels of stem chorionic villi. In view of the vasodilator action of 
      CO and NO, the HO and NOS systems may play key roles in the regulation of 
      placental haemodynamics.
FAU - Odrcich, M J
AU  - Odrcich MJ
AD  - Department of Pharmacology and Toxicology, Faculty of Health Sciences, Queen's 
      University, Kingston, Ontario, Canada.
FAU - Graham, C H
AU  - Graham CH
FAU - Kimura, K A
AU  - Kimura KA
FAU - McLaughlin, B E
AU  - McLaughlin BE
FAU - Marks, G S
AU  - Marks GS
FAU - Nakatsu, K
AU  - Nakatsu K
FAU - Brien, J F
AU  - Brien JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - 42VZT0U6YR (Heme)
RN  - 7U1EE4V452 (Carbon Monoxide)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (heme oxygenase-2)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Body Weight/physiology
MH  - Carbon Monoxide/analysis
MH  - Chorionic Villi/enzymology
MH  - Chromatography, Gas
MH  - Female
MH  - Gestational Age
MH  - Guinea Pigs
MH  - Heme/chemistry
MH  - Heme Oxygenase (Decyclizing)/*metabolism
MH  - Heme Oxygenase-1
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Isoenzymes/*metabolism
MH  - Membrane Proteins
MH  - Microsomes/enzymology
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type III
MH  - Placenta/blood supply/*enzymology
MH  - Pregnancy
EDAT- 1998/10/20 00:00
MHDA- 1998/10/20 00:01
CRDT- 1998/10/20 00:00
PHST- 1998/10/20 00:00 [pubmed]
PHST- 1998/10/20 00:01 [medline]
PHST- 1998/10/20 00:00 [entrez]
AID - S0143-4004(98)91044-X [pii]
AID - 10.1016/s0143-4004(98)91044-x [doi]
PST - ppublish
SO  - Placenta. 1998 Sep;19(7):509-16. doi: 10.1016/s0143-4004(98)91044-x.