PMID- 9778842
OWN - NLM
STAT- MEDLINE
DCOM- 19981116
LR  - 20061115
IS  - 0022-2720 (Print)
IS  - 0022-2720 (Linking)
VI  - 191
IP  - Pt 2
DP  - 1998 Aug
TI  - Energy-filtered cryotransmission electron microscopy of liposomes prepared from 
      human stratum corneum lipids.
PG  - 177-86
AB  - We used cryo-TEM to examine the morphology of vesicles formed from lipids of the 
      human stratum corneum (hSC). Human stratum corneum lipid liposomes (hSCLLs) were 
      prepared in buffer at various pH values, using different preparation methods 
      (film method, extrusion, ultrasonication, detergent dialysis). The morphology of 
      hSCLLs at pH 7.4 differed markedly from that of liposomes formed by 
      phospholipids, showing folds, stacks and membrane thickening. At pH 5.0, 
      corresponding to natural conditions at the skin surface, membrane structures are 
      essentially the same as those prepared at pH 7.4. Sharp edges in hSCLLs, 
      branching membranes and stable membrane stacks were explained by the presence of 
      ceramides, the major components and structural elements of human stratum corneum 
      lipids (hSCLs). Thickened areas in the membranes may be caused by the local 
      accumulation of triacylglycerols and cholesterol esters in the hydrophobic 
      interior of the bilayer.
FAU - Schmidtgen, M C
AU  - Schmidtgen MC
AD  - Department of Pharmaceutical Technology, Albert-Ludwigs-University, Freiburg, 
      Germany.
FAU - Drechsler, M
AU  - Drechsler M
FAU - Lasch, J
AU  - Lasch J
FAU - Schubert, R
AU  - Schubert R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Microsc
JT  - Journal of microscopy
JID - 0204522
RN  - 0 (Ceramides)
RN  - 0 (Lipids)
RN  - 0 (Liposomes)
SB  - IM
MH  - Ceramides/chemistry
MH  - Cryoelectron Microscopy/*methods
MH  - Epidermis/chemistry/*ultrastructure
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lipids/*chemistry
MH  - Liposomes/*ultrastructure
EDAT- 1998/10/21 00:00
MHDA- 1998/10/21 00:01
CRDT- 1998/10/21 00:00
PHST- 1998/10/21 00:00 [pubmed]
PHST- 1998/10/21 00:01 [medline]
PHST- 1998/10/21 00:00 [entrez]
PST - ppublish
SO  - J Microsc. 1998 Aug;191(Pt 2):177-86.