PMID- 9779588
OWN - NLM
STAT- MEDLINE
DCOM- 19981214
LR  - 20061115
IS  - 0011-4529 (Print)
IS  - 0011-4529 (Linking)
VI  - 93
IP  - 374
DP  - 1998
TI  - Amplification of telomeric DNA and the extent of karyotypic evolution.
PG  - 141-6
AB  - The distribution of telomeric DNA in the genomes of the antelope ground squirrel, 
      Ammospermophilus harrisii (family Sciuridae; 2n = 32) and the African 
      black-footed cat, Felis nigripes (family Felidae; 2n = 38) were compared by 
      fluorescence in situ hybridization (FISH) technique. These two mammalian species 
      have the highest and the lowest amount of C-banded regions, respectively. FISH 
      preparations with the human telomeric DNA probe showed that all C-banded segments 
      in the A. harrisii chromosomes, except a few intercalary segments, were 
      hybridizing with this DNA. F. nigripes showed hybridization only on the termini 
      of each chromosome, and the C-banded regions did not hybridize with telomeric DNA 
      on FISH analysis. The C-banded chromosomal arms in another rodent species, 
      Peromyscus eremicus (family Cricetidae; 2n = 48), when hybridized with human 
      telomeric DNA showed signals only in the termini of chromosomes but not in the 
      heterochromatic arms. These observations indicate that not all C-banded regions 
      in rodent species are telomeric DNA. The amplification of telomeric DNA in 
      relation to speciation is discussed.
FAU - Pathak, S
AU  - Pathak S
AD  - Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Dolhonde, J A
AU  - Dolhonde JA
FAU - Multani, A S
AU  - Multani AS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytobios
JT  - Cytobios
JID - 0207227
RN  - 0 (Heterochromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Centromere/chemistry
MH  - Chromosome Banding
MH  - DNA/*genetics
MH  - *Evolution, Molecular
MH  - *Gene Amplification
MH  - Heterochromatin/*physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mammals/*genetics
MH  - Rodentia/genetics
MH  - Species Specificity
MH  - Telomere/*chemistry
EDAT- 1998/10/21 00:00
MHDA- 1998/10/21 00:01
CRDT- 1998/10/21 00:00
PHST- 1998/10/21 00:00 [pubmed]
PHST- 1998/10/21 00:01 [medline]
PHST- 1998/10/21 00:00 [entrez]
PST - ppublish
SO  - Cytobios. 1998;93(374):141-6.