PMID- 9780243
OWN - NLM
STAT- MEDLINE
DCOM- 19981210
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 178
IP  - 5
DP  - 1998 Nov
TI  - Early diagnosis of primary human herpesvirus 6 infection in childhood: serology, 
      polymerase chain reaction, and virus load.
PG  - 1250-6
AB  - Qualitative and quantitative polymerase chain reaction (PCR) for human 
      herpesvirus 6 (HHV-6) DNA in whole blood and plasma was correlated with serology 
      and clinical assessment in 143 children hospitalized for undifferentiated febrile 
      illness to evaluate options for diagnosis of primary HHV-6 infection on the acute 
      blood specimen. PCR and serology for HHV-7 were done in parallel to define 
      serologic cross-reactions. Using HHV-6 seroconversion as the reference standard, 
      detection of HHV-6 DNA in whole blood in the absence of antibody in the plasma 
      was the most reliable evidence of primary HHV-6 infection. Detection of HHV-6 DNA 
      in plasma and a high virus load in whole blood (>3.3 log10 copies/5 microL) had a 
      sensitivity of 90% and 100%, respectively, in diagnosing primary HHV-6 infection. 
      However, both were occasionally found in patients with other infections, possibly 
      associated with HHV-6 reactivation. Maternal antibody may confound interpretation 
      of serology in patients under 3 months of age.
FAU - Chiu, S S
AU  - Chiu SS
AD  - Department of Pediatrics and Microbiology, University of Hong Kong.
FAU - Cheung, C Y
AU  - Cheung CY
FAU - Tse, C Y
AU  - Tse CY
FAU - Peiris, M
AU  - Peiris M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Antibodies, Viral/analysis
MH  - Child, Preschool
MH  - DNA, Viral/analysis
MH  - Exanthema Subitum/*diagnosis/virology
MH  - Herpesvirus 6, Human/genetics/isolation & purification
MH  - Herpesvirus 7, Human/genetics/isolation & purification
MH  - Humans
MH  - Immunoglobulin G/analysis
MH  - Infant
MH  - Polymerase Chain Reaction
MH  - *Viral Load
EDAT- 1998/10/21 02:04
MHDA- 2000/03/21 09:00
CRDT- 1998/10/21 02:04
PHST- 1998/10/21 02:04 [pubmed]
PHST- 2000/03/21 09:00 [medline]
PHST- 1998/10/21 02:04 [entrez]
AID - JID980039 [pii]
AID - 10.1086/314432 [doi]
PST - ppublish
SO  - J Infect Dis. 1998 Nov;178(5):1250-6. doi: 10.1086/314432.