PMID- 9781840
OWN - NLM
STAT- MEDLINE
DCOM- 19981224
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 21
IP  - 9
DP  - 1998 Sep
TI  - Characterization of an apoptosis-inducing factor in Habu snake venom as a 
      glycyrrhizin (GL)-binding protein potently inhibited by GL in vitro.
PG  - 924-7
AB  - By means of successive heparin-affinity and glycyrrhizin (GL)-affinity column 
      chromatographies (HPLC), a 55 kDa GL-binding protein (gp55) was purified to 
      apparent homogeneity from the Superdex P-I fraction of Habu snake venom. This 
      gp55 was identified as an apoxin I-like protein, because (i) its 20 N-terminal 
      amino acid residues (AHDRNPLEEYFRETDYEEFL) are 95% identical with the 
      corresponding sequence of apoxin I (apoptosis-inducing factor, approx. 55 kDa) in 
      the venom of the western diamondback rattlesnake; and (ii) L-amino acid oxidase 
      (LAO) activity of gp55 is detected when incubated with L-leucine, but not with 
      D-leucine. GL inhibited the LAO activity of gp55 in a dose-dependent manner, but 
      had no effect on the activity of a 65 kDa LAO also purified from Habu snake 
      venom. In addition, GL reduced the ability of gp55 to induce the hemolysis of 
      sheep red blood cells. These results suggest that GL is a potent inhibitor of 
      apoxin I-like proteins in harmful snake venoms.
FAU - Abe, Y
AU  - Abe Y
AD  - Laboratory of Genetical Biochemistry, Kitasato University School of Allied Health 
      Sciences, Sagamihara, Japan.
FAU - Shimoyama, Y
AU  - Shimoyama Y
FAU - Munakata, H
AU  - Munakata H
FAU - Ito, J
AU  - Ito J
FAU - Nagata, N
AU  - Nagata N
FAU - Ohtsuki, K
AU  - Ohtsuki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Carrier Proteins)
RN  - 0 (Crotalid Venoms)
RN  - 0 (Viper Venoms)
RN  - 0 (glycyrrhizin-binding protein, Trimeresurus flavoviridis)
RN  - 0 (glycyrrhizin-binding proteins)
RN  - 6FO62043WK (Glycyrrhizic Acid)
RN  - 9005-49-6 (Heparin)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 1.4.3.2 (L-Amino Acid Oxidase)
SB  - IM
MH  - Amino Acid Oxidoreductases/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Carrier Proteins/antagonists & inhibitors/*isolation & purification/*pharmacology
MH  - Chromatography, Affinity
MH  - Chromatography, High Pressure Liquid
MH  - Crotalid Venoms/*chemistry
MH  - Glycyrrhizic Acid/*metabolism/pharmacology
MH  - Hemolysis/drug effects
MH  - Heparin
MH  - L-Amino Acid Oxidase
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Sheep
MH  - Trimeresurus
MH  - *Viper Venoms
EDAT- 1998/10/22 00:00
MHDA- 1998/10/22 00:01
CRDT- 1998/10/22 00:00
PHST- 1998/10/22 00:00 [pubmed]
PHST- 1998/10/22 00:01 [medline]
PHST- 1998/10/22 00:00 [entrez]
AID - 10.1248/bpb.21.924 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1998 Sep;21(9):924-7. doi: 10.1248/bpb.21.924.