PMID- 9782124
OWN - NLM
STAT- MEDLINE
DCOM- 19981117
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 188
IP  - 8
DP  - 1998 Oct 19
TI  - Antigen-dependent and -independent Ca2+ responses triggered in T cells by 
      dendritic cells compared with B cells.
PG  - 1473-84
AB  - Dendritic cells (DCs) are much more potent antigen (Ag)-presenting cells than 
      resting B cells for the activation of naive T cells. The mechanisms underlying 
      this difference have been analyzed under conditions where ex vivo DCs or B cells 
      presented known numbers of specific Ag-major histocompatibility complex (MHC) 
      complexes to naive CD4(+) T cells from T cell antigen receptor (TCR) transgenic 
      mice. Several hundred Ag-MHC complexes presented by B cells were necessary to 
      elicit the formation of a few T-B conjugates with small contact zones, and the 
      resulting individual T cell Ca2+ responses were all-or-none. In contrast, 
      Ag-specific T cell Ca2+ responses can be triggered by DCs bearing an average of 
      30 Ag-MHC complexes per cell. Formation of T-DC conjugates is Ag-independent, but 
      in the presence of the Ag, the surface of the contact zone increases and so does 
      the amplitude of the T cell Ca2+ responses. These results suggest that Ag is 
      better recognized by T cells on DCs essentially because T-DC adhesion precedes Ag 
      recognition, whereas T-B adhesion requires Ag recognition. Surprisingly, we also 
      recorded small Ca2+ responses in T cells interacting with unpulsed DCs. Using DCs 
      purified from MHC class II knockout mice, we provide evidence that this signal is 
      mostly due to MHC-TCR interactions. Such an Ag-independent, MHC-triggered calcium 
      response could be a survival signal that DCs but not B cells are able to deliver 
      to naive T cells.
FAU - Delon, J
AU  - Delon J
AD  - Laboratoire d'Immunologie Cellulaire, Centre National de la Recherche 
      Scientifique UMR 7627, CERVI, 75013 Paris, France.
FAU - Bercovici, N
AU  - Bercovici N
FAU - Raposo, G
AU  - Raposo G
FAU - Liblau, R
AU  - Liblau R
FAU - Trautmann, A
AU  - Trautmann A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antigens)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens/*physiology
MH  - B-Lymphocytes/*physiology
MH  - Calcium/*metabolism
MH  - Cell Communication
MH  - Dendritic Cells/*physiology
MH  - Lymphocyte Activation
MH  - Major Histocompatibility Complex/physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Receptors, Antigen, T-Cell/physiology
MH  - T-Lymphocytes/*physiology
PMC - PMC2213410
EDAT- 1998/10/23 00:00
MHDA- 1998/10/23 00:01
PMCR- 1999/04/19
CRDT- 1998/10/23 00:00
PHST- 1998/10/23 00:00 [pubmed]
PHST- 1998/10/23 00:01 [medline]
PHST- 1998/10/23 00:00 [entrez]
PHST- 1999/04/19 00:00 [pmc-release]
AID - 10.1084/jem.188.8.1473 [doi]
PST - ppublish
SO  - J Exp Med. 1998 Oct 19;188(8):1473-84. doi: 10.1084/jem.188.8.1473.